Stretching apparatus and method for aligning microfibrils

Patent metadata

Field	Value
Publication number	US-9803167-B2
Application number	US-201514950710-A
Country	US
Kind code	B2
Filing date	Nov 24, 2015
Priority date	Nov 28, 2014
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

The present disclosure relates to stretching apparatus and method for aligning microfibrils. Specifically, the present disclosure provides an apparatus for aligning microfibrils along a single direction, which includes: a first elastic substrate onto which a composition containing microfibrils is loaded; and a stretching module which stretches the width of the elastic substrate. In accordance with the apparatus the present disclosure, microfibrils or cells may be aligned along a particular direction simply by pulling and then releasing the elastic substrate. The present disclosure is also useful for culturing of the aligned cells because the physiological activity of the cells can be maintained and cytotoxicity can be prevented.

First claim

What is claimed is:

1. An apparatus for aligning microfibrils along a single direction, comprising:
a first elastic substrate onto which a composition comprising microfibrils is loaded; and
a stretching module which stretches the width of the elastic substrate,
wherein the stretching module comprises: a handle which grips both ends of the first elastic substrate; a stretcher which stretches the first elastic substrate by operating the handle; and a controller which controls the movement of the stretcher, and,
wherein the stretching module further comprises a support having a height corresponding to the difference between the height of the first elastic substrate and the height of the handle.

2. The apparatus according to claim 1 , wherein the first elastic substrate comprises a well in which the composition containing microfibrils is loaded.

3. The apparatus according to claim 1 , wherein the first elastic substrate comprises an elastomer.

4. The apparatus according to claim 3 , wherein the elastomer is one or more selected from a group consisting of natural polyisoprene, synthetic polyisoprene, polybutadiene, chloroprene rubber, butyl rubber, styrene-butadiene rubber, nitrile rubber, ethylene propylene rubber, ethylene propylene diene rubber, epichlorohydrin rubber, polyacrylic rubber, silicone rubber, fluorosilicone rubber, fluoroelastomers, polyether block amides, chlorosulfonated polyethylene, ethylene-vinyl acetate and polydimethylsiloxane.

5. The apparatus according to claim 1 , wherein the microfibril is one or more synthetic polymer fiber selected from the group consisting of nylon, polyacrylic acid, polycarbonate, polyurethane, poly(ethylene-vinyl acetate), polystyrene, polyvinyl alcohol, cellulose acetate and polyethylene oxide or one or more natural polymer fiber selected from the group consisting of elastin, gelatin, fibrinogen, fibrin, alginate, cellulose, silk fibroin, chitosan, laminin, actin and collagen.

6. The apparatus according to claim 1 , wherein the composition comprising microfibrils further comprises one or more types of cells.

7. The apparatus according to claim 6 , wherein the cell is a neuron, a glial cell, a muscle cell, a solid cancer cell, a mesenchymal stem cell or a fibroblast.

8. The apparatus according to claim 1 , wherein the apparatus comprises a second elastic substrate which is assembled on or disassembled on the first elastic substrate.

9. The apparatus according to claim 8 , wherein the second elastic substrate comprises two or more holes for loading the composition comprising microfibrils onto the first elastic substrate.

10. The apparatus according to claim 1 , wherein the controller stretches the first elastic substrate by 5-80% of its width by operating the stretcher.

11. The apparatus according to claim 10 , wherein the controller, after stretching the width of the first elastic substrate by operating the stretcher, maintains the stretched state for 1-10 minutes when the composition comprising microfibrils is loaded onto the first elastic substrate and then restores the first elastic substrate.

12. The apparatus according to claim 1 , wherein the stretching module further comprises a fixture which is disposed between the support and maintains the stretched state of the first elastic substrate.

13. The apparatus according to claim 1 , wherein the microfibrils are aligned to be perpendicular to the stretching direction of the elastic substrate.

14. The apparatus according to claim 1 , wherein the first elastic substrate further comprises an adhesive coated on its surface.

15. The apparatus according to claim 14 , wherein the adhesive is one or more selected from the group consisting of glutaraldehyde, polyethylenimine, poly-L-lysine, poly-D-lysine and polydopamine.

Assignees

• Korea Inst Sci & Tech

Inventors

• Choi Nakwon
• Hur Eun-Mi
• Kim Sohyun
• Im Sun-Kyoung
• Yoon Eui Sung
• Lee Changjoon Justin

Classifications

• C12M35/04 Primary

  Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli · CPC title

• C12M3/003

  for culture in eggs · CPC title

• C08J5/00

  Manufacture of articles or shaped materials containing macromolecular substances (manufacture of semi-permeable membranes B01D67/00 - B01D71/00) · CPC title

• C07K14/78

  Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] · CPC title

• B29C55/20

  Edge clamps · CPC title