Probe for a biological specimen and labelling method and screening method using the probe

Patent metadata

Field	Value
Publication number	US-9801960-B2
Application number	US-201514624558-A
Country	US
Kind code	B2
Filing date	Feb 17, 2015
Priority date	Dec 25, 2008
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

Provided is a novel probe for a biological specimen for labelling by itself and clearly visualizing one of a specific cell and a specific cell organ in a living body, the probe having excellent spectral characteristics and exhibiting excellent storage stability. The probe for a biological specimen contains, as an active agent, at least one kind of compound represented by a general formula (I).

First claim

The invention claimed is:

1. A labelling method, wherein one of staining and differential staining is performed by bringing a biological specimen into contact with a composition comprising a probe for a biological specimen comprising, as an active agent, at least one member among the following formulae

2. A labelling method according to claim 1 , wherein the biological specimen is used in vivo.

3. A method, comprising the steps of applying to a sample a composition for dyeing a biological specimen; and
determining whether or not said biological specimen is dyed, wherein
said composition comprises a probe for said biological specimen comprising, as an active agent, at least one member among the following formulae

4. A method according to claim 3 , wherein the biological specimen is a small bony fish.

5. A method according to claim 4 , wherein the small bony fish comprises Zebrafish.

6. A labelling method according to claim 1 , wherein the composition further comprises dimethyl sulfoxide.

7. A method according to claim 3 , wherein the composition further comprises dimethyl sulfoxide.

8. A labelling method according to claim 1 , which is carried out on the biological specimen in vitro.

9. A labelling method according to claim 1 , which is carried out on the biological specimen ex vivo.

10. A labelling method according to claim 2 , wherein the labelling method is performed in vivo with an individual organism, a microorganism, protozoan, a biological tissue, a biological tissue section, a human cell or an animal cell.

11. A method according to claim 8 , wherein the labelling method is performed in vitro with a biological tissue, a biological tissue section, a human cell or an animal cell.

12. A method according to claim 9 , wherein the labelling method is performed ex vivo with a biological tissue, a biological tissue section, a human cell or an animal cell.

Assignees

• Canon Kk

Inventors

• Shintou Taichi
• Miyazaki Takeshi
• Hirose Masashi
• Okubo Taketoshi
• Watanabe Kohei
• Nomoto Tsuyoshi
• Tanaka Toshio
• Nishimura Yuhei
• Shimada Yasuhito
• Nishimura Norihiro

Classifications

• A61K49/006

  Biological staining of tissues in vivo, e.g. methylene blue or toluidine blue O administered in the buccal area to detect epithelial cancer cells, dyes used for delineating tissues during surgery · CPC title

• A61K49/10

  Organic compounds · CPC title

• G01N2333/4603

  from fish · CPC title

• C07D403/06

  linked by a carbon chain containing only aliphatic carbon atoms · CPC title

• G01N2500/10

  involving cells · CPC title