Agglutination enhancer

Patent metadata

Field	Value
Publication number	US-9797886-B2
Application number	US-201214123899-A
Country	US
Kind code	B2
Filing date	Jun 4, 2012
Priority date	Jun 7, 2011
Publication date	Oct 24, 2017
Grant date	Oct 24, 2017

Abstract

The purpose of the present invention is to provide an agglutination enhancer which shows superior agglutination enhancing effect to those of conventional immunoagglutination enhancers, and the present invention relates to an agglutination enhancer for an immunoagglutination measurement method which comprises a polymer having a monomer unit shown by the following general formula [1]:

(Wherein R 1 represents a hydrogen atom or a methyl group; R 2 and R 3 independently represent a methyl group or an ethyl group, respectively; X represents —NH— or an oxygen atom; n represents an integer of 1 to 6; and m represents an integer of 1 to 3), and an immunoagglutination measurement method in which, in the coexistence of the above-described agglutination enhancer for immunoagglutination measurement method, an antibody against analyte or an antigen for the analyte is brought into contact with the analyte to cause an antigen-antibody reaction.

First claim

The invention claimed is:

1. A method of measuring agglutination, which comprises
bringing an antibody or an antigen against an analyte into contact with the analyte, to cause an antigen-antibody reaction, in the presence of a polymer that is either
(a) a homopolymer consisting of a monomer unit of formula [1]:

wherein R 1 is a hydrogen atom or a methyl group; R 2 and R 3 are a methyl group, X is —NH— or an oxygen atom; n is an integer of 2 to 4; and m is an integer of 1
or
(b) a copolymer consisting of
(i) a monomer unit of formula [1]:

wherein R 1 is a hydrogen atom or a methyl group; R 2 and R 3 are a methyl group, X is —NH— or an oxygen atom; n is an integer of 2 to 4; and m is an integer of 1, and
(ii) a monomer unit of formula [2]:

wherein R 4 is a hydrogen atom or a methyl group, and k is an integer of 4 to 8, to generate agglutination and form particles from the antigen-antibody reaction, and
measuring the degree of agglutination of the particles based on a change of scattered light or transmitted light.

2. The method according to claim 1 , wherein the analyte is C-reactive protein (CRP), serum ferritin (Fer), prostate specific antigen (PSA), or creatine kinase-MB (CK-MB).

3. The method according to claim 1 , wherein the content of the monomer unit of formula [1] in the copolymer is 50% by mole or more and less than 100% by mole.

4. The method according to claim 1 , wherein the polymer has a weight-average molecular weight of 50,000 to 3,000,000 g/mol.

5. A method of measuring agglutination, which comprises
bringing an antibody or an antigen against an analyte into contact with the analyte, to cause an antigen-antibody reaction, in the presence of a polymer that is either
(a) a homopolymer consisting of a monomer unit that is

or
(b) a copolymer consisting of
(i) a monomer unit that is

and
(ii) a monomer unit of formula [2-1-1]:

to generate agglutination and form particles from the antigen-antibody reaction, and
measuring the degree of agglutination of the particles based on a change of scattered light or transmitted light.

Assignees

• Yamamoto Naoyuki
• Masuda Tsutomu
• Wako Pure Chem Ind Ltd

Inventors

• Yamamoto Naoyuki
• Masuda Tsutomu

Classifications

• G01N33/542 Primary

  with steric inhibition or signal modification, e.g. fluorescent quenching · CPC title

• C08F220/60

  containing nitrogen in addition to the carbonamido nitrogen · CPC title

• C08F220/36

  containing oxygen in addition to the carboxy oxygen {, e.g. 2-N-morpholinoethyl (meth)acrylate or 2-isocyanatoethyl (meth)acrylate} · CPC title

• G01N33/5306 Primary

  Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding · CPC title

• C08F220/34

  Esters containing nitrogen {, e.g. N,N-dimethylaminoethyl (meth)acrylate} · CPC title