Monoclonal antibody recognizing sialylated sugar chains

Patent metadata

Field	Value
Publication number	US-9796785-B2
Application number	US-201414888147-A
Country	US
Kind code	B2
Filing date	May 2, 2014
Priority date	May 2, 2013
Publication date	Oct 24, 2017
Grant date	Oct 24, 2017

Abstract

The purpose of the present invention is to provide a novel monoclonal antibody having high affinity and that strictly recognizes, as a sugar chain epitope, only a “Siaα2,6Galβ1,4GlcNAc (6′-Sialyl-LacNAc): CDw75” sugar chain structure, being a molecular target for diagnosis of the malignancy of tumors. An anti-CDw75 monoclonal antibody is provided that recognizes “CDw75” sugar chain structures but does not recognize similar sugar chain structures indicated by “Galβ1,4GlcNAc”, “Siaα2,3Galβ1,4GlcNAc”, or “Siaα2,6Galβ1,4Glc”, by using a glycolipid antigen bonding a carrier lipid compound “HOCH 2 CH(NH—CO—(CH 2 ) 22 —CH 3 )—(CH 2 ) 9 —CH 3 (C12L)” developed by the inventors to a “CDw75” sugar chain. The obtained anti-CDw75 monoclonal antibody is an excellent detection drug for B-cell lymphoma, gastric cancer, or colorectal cancer, an excellent diagnostic agent for tumor malignancy, etc., an excellent treatment agent for B-cell lymphoma, gastric cancer, or colorectal cancer, and an excellent prevention/treatment drug for influenza.

First claim

The invention claimed is:

1. An anti-CDw75 monoclonal antibody produced by hybridoma FR9 deposited under Accession No. NITE BP-01516, or a fragment thereof.

2. A composition comprising the anti-CDw75 monoclonal antibody or a fragment thereof according to claim 1 , and a pharmaceutically acceptable carrier.

3. A kit for determining morbidity and/or tumor malignancy of gastric cancer or colorectal cancer, the kit comprising as an effective component the anti-CDw75 monoclonal antibody or a fragment thereof according to claim 1 .

4. Hybridoma FR9 deposited under Accession No. NITE BP-01516 which produces anti-CDw75 monoclonal antibody.

Assignees

• Nat Inst Advanced Ind Science & Tech

Inventors

• Okuda Tetsuya

Classifications

• C12Y204/99001

  Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1) · CPC title

• C07K2317/14

  Specific host cells or culture conditions, e.g. components, pH or temperature · CPC title

• C07K2317/92

  Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value · CPC title

• C07K16/2896

  against molecules with a "CD"-designation, not provided for elsewhere · CPC title

• G01N2440/38

  addition of carbohydrates, e.g. glycosylation, glycation · CPC title