Method for detecting pancreatic cancer

Patent metadata

Field	Value
Publication number	US-9796775-B2
Application number	US-201214236807-A
Country	US
Kind code	B2
Filing date	Aug 3, 2012
Priority date	Aug 4, 2011
Publication date	Oct 24, 2017
Grant date	Oct 24, 2017

Abstract

This invention relates to a method for detecting pancreatic cancer using novel tumor markers. Specifically, the invention provides a method for detecting pancreatic cancer comprising measuring the presence or an amount of a polypeptide having an reactivity of binding via an antigen-antibody reaction to an antibody against CAPRIN-1 protein in a sample separated from a subject, and to a reagent or kit for detecting pancreatic cancer comprising a CAPRIN-1 protein or a fragment thereof, an antibody against the same, or a polynucleotide encoding the same.

First claim

The invention claimed is:

1. A method for detecting and treating pancreatic cancer in a human subject, comprising the following steps:
contacting a sample from the human subject with an antibody that specifically binds a human CAPRIN-1 protein expressed on the surface of a pancreatic cancer cell;
measuring an amount of a polypeptide bound to the antibody in the sample;
comparing the measured amount of the polypeptide with an amount of a control;
determining that the human subject has a pancreatic cancer when the amount of the polypeptide is higher than the amount of the control; and
treating the human subject detennined to have pancreatic cancer by administration of an antibody that specifically binds a human CAPRIN-1 protein expressed on the surface of a pancreatic cancer cell,
wherein the pancreatic cancer is a human CAPRIN-1 expressing pancreatic cancer, and
wherein the sample is a pancreatic tissue or pancreatic cell sample.

2. The method according to claim 1 , wherein the polypeptide to be measured is a human CAPRIN-1 protein consisting of an amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4.

3. The method according to claim 1 , wherein the measurement is an immunological assay.

4. The method according to claim 1 , wherein the antibody specifically binds a region consisting of amino acid residues 50-98 or 233-305 in an amino acid sequence of SEQ ID NO: 2.

5. The method according to claim 1 , wherein the antibody is a monoclonal antibody.

6. The method according to claim 1 , wherein the antibody is a monoclonal antibody that specifically binds a region consisting of amino acid residues 50-98 or 233-305 in an amino acid sequence of SEQ ID NO: 2.

Assignees

• Ido Takayoshi
• Okano Fumiyoshi
• Toray Industries

Inventors

• Ido Takayoshi
• Okano Fumiyoshi

Classifications

• G01N33/57525 Primary

  of the liver or pancreas · CPC title

• C07K2317/34

  Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues · CPC title

• C07K14/47 Primary

  from mammals · CPC title

• C07K16/18 Primary

  against material from animals or humans · CPC title

• C07K16/30

  from tumour cells · CPC title