Compositions and methods relating to nucleic acid nano- and micro-technology

Patent metadata

Field	Value
Publication number	US-9796749-B2
Application number	US-201214237001-A
Country	US
Kind code	B2
Filing date	Aug 2, 2012
Priority date	Aug 5, 2011
Publication date	Oct 24, 2017
Grant date	Oct 24, 2017

Abstract

The invention provides nucleic acid structures of controlled size and shape, comprised of a plurality of oligonucleotides, and methods for their synthesis. The structures are formed, at least in part, by the self-assembly of single stranded oligonucleotides. The location of each oligonucleotide in the resultant structure is known. Accordingly, the structures may be modified with specificity.

First claim

What is claimed is:

1. A nucleic acid structure comprising
a plurality of oligonucleotides, wherein at least one oligonucleotide of the plurality comprises at least four domains, wherein each of the four domains is annealed to a complementary domain of another oligonucleotide of the plurality to form at least two parallel double helices within the nucleic acid structure, wherein oligonucleotides having single-stranded poly(T), poly(A) or poly(C) domains that each have a length of 4-22 nucleotides are present at borders around the nucleic acid structure.

2. The nucleic acid structure of claim 1 , wherein at least one double helix within the nucleic acid structure comprises 2 or more unique domains.

3. The nucleic acid structure of claim 1 , wherein at least 50% of the double helices within the nucleic acid structure comprise one or more unique domains.

4. The nucleic acid structure of claim 1 , wherein the nucleic acid structure comprises at least 5, at least 10, or at least 20 parallel double helices.

5. The nucleic acid structure of claim 1 , wherein a first subset of oligonucleotides of the plurality comprises 2 domains and a second subset of oligonucleotides of the plurality comprises 4 domains.

6. The nucleic acid structure of claim 1 , wherein the oligonucleotides are 21-104 nucleotides in length.

7. The nucleic acid structure of claim 1 , wherein the oligonucleotides are DNA oligonucleotides.

8. The nucleic acid structure of claim 1 , wherein the oligonucleotides are L-DNA oligonucleotides.

9. The nucleic acid structure of claim 1 , wherein at least one of the double helices within the nucleic acid structure is unique.

10. The nucleic acid structure of claim 9 , wherein the nucleic acid structure comprises 2 or more unique double helices.

11. The nucleic acid structure of claim 9 , wherein at least 50% of the double helices are unique.

12. The nucleic acid structure of claim 9 , wherein at least 50% of the double helices comprise one or more unique domains.

13. The nucleic acid structure of claim 9 , wherein the nucleic acid structure comprises at least 5, at least 10, or at least 20 parallel double helices.

14. The nucleic acid structure of claim 9 , wherein a first subset of oligonucleotides of the plurality comprises 2 domains and a second subset of oligonucleotides of the plurality comprises 4 domains.

15. The nucleic acid structure of claim 1 , wherein at least one oligonucleotide in the nucleic acid structure is unique.

16. A composition comprising a plurality of nucleic acid structures of claim 1 , wherein at least 50% of the nucleic acid structures of the plurality are identical.

17. A composite nucleic acid structure comprising at least two nucleic acid structures of claim 1 , conjugated to each other through a spacer-linker.

18. The nucleic acid structure of claim 1 , wherein the nucleic acid structure has an internal void pattern.

19. The nucleic acid structure of claim 1 , wherein the nucleic acid structure further comprises oligonucleotides having a handle domain.

20. The nucleic acid structure of claim 19 , wherein the oligonucleotides having a handle domain are present at borders of the nucleic acid structure.

21. The nucleic acid structure of claim 19 , wherein the handle domain is bound to an oligonucleotide conjugated to a moiety of interest.

22. The nucleic acid structure of claim 1 , wherein the single-stranded poly(T), poly(A) or poly(C) domains have a length of 10 nucleotides.

23. The nucleic acid structure of claim 1 , wherein the single-stranded poly(T), poly(A) or poly(C) domains have a length of 11 nucleotides.

Assignees

• Yin Peng
• Wei Diming
• Harvard College

Inventors

• Yin Peng
• Wei Diming

Classifications

• C12Q2525/197

  incorporating a spacer/coupling moiety · CPC title

• C12Q2525/161

  incorporating target specific and non-target specific sites · CPC title

• C12Q2563/157

  Nanotubes or nanorods · CPC title

• C12Q2565/631

  being a biochannel or pore · CPC title

• C12Q2525/185

  incorporating bases where the precise position of the bases in the nucleic acid string is important · CPC title