Spatial sequestration of dynamic nucleic acid circuits

Patent metadata

Field	Value
Publication number	US-9796748-B2
Application number	US-201214114961-A
Country	US
Kind code	B2
Filing date	May 2, 2012
Priority date	May 2, 2011
Publication date	Oct 24, 2017
Grant date	Oct 24, 2017

Abstract

The invention provides systems and methods for spatial sequestration of elements in nucleic acid circuits.

First claim

What is claimed is:

1. A synthetic system comprising
(a) a first three-dimensional nucleic acid nanostructure shaped in the form of a container comprising walls, an interior surface and an exterior surface,
(b) a first tethered nucleic acid attached to the interior surface of the first three-dimensional nucleic acid nanostructure and partially hybridized to a first output signal nucleic acid, wherein the first tethered nucleic acid contains a toehold domain that is not complementary to the first output signal nucleic acid,
(c) a second three-dimensional nucleic acid nanostructure shaped in the form of a container comprising walls, an interior surface and an exterior surface, and
(d) a second tethered nucleic acid attached to the interior surface of the second three-dimensional nucleic acid nanostructure and partially hybridized to a second output signal nucleic acid, wherein the second tethered nucleic acid contains a toehold domain that is not complementary to the second output signal nucleic acid, and
wherein the toehold domain of the second tethered nucleic acid is complementary to a domain of the first output signal nucleic acid.

2. The system of claim 1 , wherein the first and second tethered nucleic acids are not integral to the first and second nucleic acid nanostructures.

3. The system of claim 1 , wherein the first and second nucleic acid nanostructures are independently selected from the group consisting of closed nucleic acid nanostructures and open nucleic acid nanostructures.

4. The system of claim 3 , wherein the open nucleic acid nanostructures comprise two open ends.

5. The system of claim 3 , wherein the closed nucleic acid nanostructures comprise a nucleic acid lock.

6. The system of claim 1 , further comprising one or more upstream and/or downstream nucleic acid nanostructures.

7. The system of claim 6 , wherein the one or more of the upstream and/or downstream nucleic acid nanostructures comprise tethered nucleic acid.

8. A method comprising
contacting a first input signal nucleic acid with the system of claim 1 in an amount effective to release the second output signal nucleic acid from the second nucleic acid nanostructure.

9. The system of claim 6 , wherein the one or more of the upstream and/or downstream nucleic acid nanostructures comprise free flowing nucleic acids.

10. The system of claim 9 , wherein the free flowing nucleic acids are output signal nucleic acids.

11. The system of claim 1 , wherein the first nucleic acid nanostructures and/or the second nucleic acid nanostructure comprises an agent.

12. The system of claim 11 , wherein the agent is or comprises a therapeutic agent.

13. The system of claim 11 , wherein the agent is or comprises a detectable marker.

14. The system of claim 1 , wherein the first nucleic acid nanostructures and/or the second nucleic acid nanostructure comprises a plurality of tethered nucleic acids.

15. The system of claim 1 , wherein the first nucleic acid nanostructures and/or the second nucleic acid nanostructure comprises a plurality of free flowing nucleic acids.

16. The system of claim 1 , wherein the length of the toehold domain of the first tethered nucleic acid is 4-12 nucleotides.

17. The system of claim 1 , wherein the length of the toehold domain of the second tethered nucleic acid is 4-12 nucleotides.

18. The system of claim 1 , wherein the first nucleic acid nanostructure is a deoxyribonucleic acid (DNA) nanostructure.

19. The system of claim 1 , wherein the second nucleic acid nanostructure is a DNA nanostructure.

Assignees

• Schaus Thomas E
• Zhang David Yu
• Sun Wei
• Yin Peng
• Harvard College

Inventors

• Schaus Thomas E
• Zhang David Yu
• Sun Wei
• Yin Peng

Classifications

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• C07H21/04 Primary

  with deoxyribosyl as saccharide radical · CPC title

• Y10S977/795

  Composed of biological material · CPC title

• C12Q1/16

  using radioactive material · CPC title