Chemically-linked nanoparticles

Patent metadata

Field	Value
Publication number	US-9795691-B2
Application number	US-201414313736-A
Country	US
Kind code	B2
Filing date	Jun 24, 2014
Priority date	Jun 24, 2013
Publication date	Oct 24, 2017
Grant date	Oct 24, 2017

Abstract

Provided herein are compositions and methods of preparing nanoparticle aggregates.

First claim

What is claimed is:

1. A nanoparticle aggregate comprising:
(i) a plurality of interior nanoparticle cores covalently bound together and enclosed by a plurality of exterior nanoparticle cores covalently bound together, wherein at least a portion of said plurality of interior nanoparticle cores are covalently bound to said plurality of exterior nanoparticle cores;
(ii) a plurality of interior polyvalent linkers covalently binding said interior nanoparticle cores together, covalently binding said exterior nanoparticle cores together, and covalently binding said portion of said interior nanoparticle cores to said exterior nanoparticle cores, wherein each of said plurality of interior polyvalent linkers comprises a central polyvalent moiety covalently bound to at least two nanoparticle linkers, each of said nanoparticle linkers covalently bound to one of said interior nanoparticle cores or one of said exterior nanoparticle cores, and wherein said plurality of interior polyvalent linkers do not bind a capping substituent to said interior nanoparticle cores;
(iii) a plurality of exterior polyvalent linkers covalently binding at least one capping substituent to said exterior nanoparticle cores, wherein each of said plurality of exterior polyvalent linkers comprises:
(a) said central polyvalent moiety covalently bound to at least one of said nanoparticle linkers, said nanoparticle linkers covalently bound to one of said exterior nanoparticle cores; and
(b) at least one capping linker covalently bound to said capping substituent, wherein said plurality of exterior polyvalent linkers comprise at least two nanoparticle linkers and capping linkers combined;

wherein the plurality of interior nanoparticle cores and the plurality of exterior nanoparticle cores are each independently titanium, zirconium, gold, silver, platinum, cerium, arsenic, iron, aluminum, carbon, boron, cadmium, selenium, zinc, silicon, or a metal oxide of any one of the foregoing; and
wherein the nanoparticle linker and the capping linker are each independently —C(O)—, —C(O)O—, —O—, —S—, —NH—, —NR 1 —, —C(O)NR 2 —, —S(O) n —, —S(O)NR 3 —, —OP(O)(OR 4 )O—, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene, an amino acid sequence, or a nucleic acid sequence;
R 1 , R 2 , R 3 , R 4 , are independently hydrogen, halogen, —N 3 , —NO 2 , —CF 3 , —CCl 3 , —CBr 3 , —CI 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 2 Cl, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —OCH 3 , —NHCNHNH 2 , substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl; and
n is 1 or 2.

2. The nanoparticle aggregate of claim 1 , wherein said plurality of interior nanoparticle cores and said plurality of exterior nanoparticle cores are chemically identical.

3. The nanoparticle aggregate of claim 2 , wherein said plurality of interior nanoparticle cores and said plurality of exterior nanoparticle cores comprise gold, silver, or platinum.

4. The nanoparticle aggregate of claim 1 , wherein said plurality of exterior nanoparticle cores and said plurality of interior nanoparticle cores are about 2 to about 20 nm in diameter.

5. The nanoparticle aggregate of claim 1 , wherein said nanoparticle linker is —C(O)—, —C(O)O—, —O—, —S—, —NH—, —NR 1 —, —C(O)NR 2 —, —S(O) n —, —S(O)NR 3 —, —OP(O)(OR 4 )O—, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene;
R 1 , R 2 , R 3 , R 4 , are independently hydrogen, halogen, —N 3 , —NO 2 , —CF 3 , —CCl 3 , —CBr 3 , —CI 3 , —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 2 Cl, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —OCH 3 , —NHCNHNH 2 , substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl; and
n is 1 or 2.

6. The nanoparticle aggregate of claim 1 , wherein said capping linker is —C(O)—, —C(O)O—, —O—, —S—, —NH—, —NR 1 —, —C(O)NR 2 —, —S(O) n —, —S(O)NR 3 —, —OP(O)(OR 4 )O—, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene;
R 1 , R 2 , R 3 , R 4 , are independently hydrogen, halogen, —N 3 , —NO 2 , —CF 3 , —CCl 3 , —CBr 3 , —CI 3 , —CN, —OH, —NH 27 —COOH, —CONH 2 , —NO 2 , —SH, —SO 2 Cl, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —OCH 3 , —NHCNHNH 2 , substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl; and
n is 1 or 2.

7. The nanoparticle aggregate of claim 1 , wherein said plurality of exterior polyvalent linkers comprise at least three nanoparticle linkers and capping linkers combined.

8. The nanoparticle aggregate of claim 1 , wherein said nanoparticle linker comprises a nanoparticle linker-cleavage site and wherein said capping linker comprises a capping linker-cleavage site.

9. The nanoparticle aggregate of claim 8 , wherein said nanoparticle linker-cleavage site and said capping linker-cleavage site are independently an ester that is recognized by an esterase.

10. The nanoparticle aggregate of claim 1 , wherein said capping substituent is substituted with a capping functional group, said capping functional group comprising a hydrophobic moiety, a hydrophilic moiety, a biological moiety, a therapeutic moiety, a targeting moiety, a water soluble polymer moiety, or a detectable moiety.

11. The nanoparticle aggregate of claim 1 , wherein said nanoparticle aggregate is about 50 nm in diameter.

12. A method for preparing a nanoparticle aggregate of claim 1 , said method comprising:
(i) contacting a plurality of nanoparticle cores with a first plurality of reactive polyvalent linkers thereby forming a plurality of polyvalent linkers covalently binding said nanoparticle cores together, wherein a first portion of said plurality of nanoparticle cores are covalently bound to together to form a plurality of interior nanoparticle cores and a second portion of said plurality of nanoparticle cores are covalently bound together to form a plurality of exterior nanoparticle cores enclosing said plurality of interior nanoparticle cores, wherein said plurality of exterior nanoparticle cores are covalently bound to a portion of said plurality of interior nanoparticle cores;
(ii) allowing a portion of said plurality of reactive polyvalent linkers to react with a portion of said exterior nanoparticle cores thereby forming a plurality of reactive exterior polyvalent linkers covalently bound to said portion of said plurality of exterior nanoparticle cores;
(iii) contacting said nanoparticle aggregate with a reactive capping substituent and allowing said reactive capping substituent to react with said plurality of reactive exterior polyvalent linkers thereby forming said nanoparticle aggregate.

13. The metho

Assignees

• Hope City

Inventors

• Berlin Jacob
• Van Haute Desiree

Classifications

• Y10T428/2982

  Particulate matter [e.g., sphere, flake, etc.] · CPC title

• A61K49/0002 Primary

  General or multifunctional contrast agents, e.g. chelated agents · CPC title

• A61K47/6941

  the form being a granulate or an agglomerate · CPC title

• A61K47/4893

  Human Necessities · mapped topic