Fluid mixing and rinsing system for a flow cytometer

Patent metadata

Field	Value
Publication number	US-9791362-B2
Application number	US-201715417131-A
Country	US
Kind code	B2
Filing date	Jan 26, 2017
Priority date	Jun 7, 2012
Publication date	Oct 17, 2017
Grant date	Oct 17, 2017

Abstract

Disclosed is a system that can mix deionized water and concentrated sheath fluid to provide sheath fluid in a flow cytometer system having a desired concentration. Flow rates are low, which substantially match the flow rate of sheath fluid through the nozzle, so that turbulence and air bubbles are not formed in the sheath fluid. The available deionized water is then used for back flushing and removal of sample cells and deposited salts from the sheath fluid.

First claim

What is claimed is:

1. A method of mixing deionized water and concentrated sheath fluid in a flow cytometer comprising:
supplying deionized water from a first container;
supplying concentrated sheath fluid from a second container;
pumping the deionized water and the concentrated sheath fluid into a reservoir at a rate that is sufficiently slow to substantially eliminate turbulence that causes bubbles to form in the reservoir; and
controlling a first valve that is connected to the first container in a first position and the second container in a second position so that the first valve is disposed in the first position until a predetermined amount of the deionized water is supplied to the reservoir and the first valve is disposed in the second position until a predetermined amount of the concentrated sheath fluid is supplied to the reservoir to produce sheath fluid having a predetermined concentration in the reservoir.

2. The method of claim 1 wherein controlling the first valve comprises:
sampling a pump control signal to generate a sampled pump control signal;
summing the sampled pump control signal when the first valve is in the first position to determine when the predetermined amount of the deionized water has been delivered to the reservoir; and
summing the sampled pump control signal when the first valve is in the second position to determine when the predetermined amount of the concentrated fluid has been delivered to the reservoir.

3. The method of claim 2 wherein the process of pumping the deionized water and the concentrated sheath fluid comprises:
pumping the deionized water and the concentrated sheath fluid at a rate that substantially matches an outflow rate of sheath fluid to the reservoir and a rate sufficient to establish, re-establish, and maintain a substantially constant level of the sheath fluid in the reservoir.

4. The method of claim 1 further comprising:
pumping the sheath fluid with a rinse pump to provide a supply of deionized water for cleaning parts that contact the sheath fluid and sample fluid in the flow cytometer;
applying the supply of deionized water to a nozzle in the flow cytometer so that the deionized water flows out of the nozzle into a waste collector to rinse the nozzle and the waste collector with the deionized water; and
applying the supply of deionized water to the nozzle to cause the deionized water to backwash an injector needle, a sample tube and a sample uptake tube to remove sheath fluid and sample particles.

5. A method for mixing deionized water in a first container and sheath fluid concentrate with a first concentration in a second container, the method comprising:
pumping, using a pump that is fluidically interposed between a valve and a pressurized reservoir, deionized water in the first container and concentrated sheath fluid in the second container into the pressurized reservoir at a flowrate that is sufficiently slow that substantially no bubbles form in the pressurized reservoir, wherein the valve has a first input that is fluidically coupled to the first container, wherein the valve has a second input that is fluidically coupled to the second container, wherein the valve is configured to allow deionized water in the first container to flow through an output to the pressurized reservoir when in a first position, and wherein the valve is configured to allow concentrated sheath fluid in the second container to flow through the output to the pressurized reservoir when in a second position; and
maintaining a concentration of a mixture of the deionized water and the concentrated sheath fluid in the pressurized reservoir such that the mixture has a concentration of sheath fluid less than the first concentration by repeatedly switching the valve between the first position, thereby supplying a first amount of deionized water by the pump to the pressurized reservoir, and the second position, thereby supplying a second amount of concentrated sheath fluid by the pump to the pressurized reservoir.

6. The method of claim 5 , further comprising flowing the mixture out of the pressurized reservoir at an outflow rate, wherein pumping the deionized water in the first container and the concentrated sheath fluid in the second container into the pressurized reservoir is at a flowrate that is substantially equal to the outflow rate out of the pressurized reservoir.

7. The method of claim 6 , further comprising:
generating, using a level sensor, a level sensor signal that is representative of a level of the mixture in the pressurized reservoir, and
changing, based on the level sensor signal from the level sensor, the flowrate of the pump.

8. The method of claim 7 , wherein changing the flowrate of the pump further comprises determining that the level of the mixture in the pressurized reservoir is below a first level and increasing, based on the determination, the flowrate of the pump.

9. The method of claim 7 , wherein changing the flowrate of the pump further comprises determining that the level of the mixture in the pressurized reservoir is above a second level and decreasing, based on the determination, the flowrate of the pump.

10. The method of claim 5 , further comprising pumping, using a rinse pump fluidically connected to the first container and fluidically interposed between a second valve and the first container, deionized water in the first container to the second valve and to a nozzle of a flow cytometer, wherein the nozzle is fluidically connected to the second valve, and wherein the second valve is fluidically interposed between the nozzle and the rinse pump.

11. The method of claim 10 , further comprising pumping, using the rinse pump, deionized water in the first container to one or more components selected from the group of: an injector needle, a sample tube, and a sample uptake tube, wherein each of the one or more components are fluidically connected to the second valve.

12. The method of claim 10 , wherein pumping, using the rinse pump, deionized water in the first container to the nozzle further comprises pumping deionized water to a waste collector fluidically connected to the nozzle, wherein the nozzle is fluidically interposed between the second valve and the waste collector.

13. The method of claim 12 , further comprising pumping, using a waste pump fluidically interposed between the waste collector and a waste container, material in the waste collector to the waste container.

14. The method of claim 5 , wherein maintaining a concentration of a mixture of the deionized water and the concentrated sheath fluid in the pressurized reservoir further comprises maintaining a concentration of a mixture that is nominally seven parts deionized water to one part concentrated sheath fluid.

15. The method of claim 5 , wherein pumping deionized water in the first container and concentrated sheath fluid in the second container into the pressurized reservoir at a flowrate further comprises a flowrate of about 8 milliliters per minute.

16. The method of claim 5 , wherein:
the first amount is about 7 milliliters of deionized water, and
the second amount is about 1 milliliter of concentrated sheath fluid.

17. The method of claim 5 , further comprising:
sampling a pump control signal;
creating a sampled pump control signal; and
summing the sampled pump control signal to determine when the first amount of deionized water has been delivered to the pressurized container and to determine when the second amount of concentrated sheath fluid has been delivered to the pressurized container.

18. The method

Assignees

• Bio Rad Laboratories Inc

Inventors

• Fox Daniel Nelson
• Gaskill-Fox Nathan Michael

Classifications

• G01N15/14 Primary

  Optical investigation techniques, e.g. flow cytometry · CPC title

• G01N15/1404 Primary

  Handling flow, e.g. hydrodynamic focusing · CPC title

• B01F23/49

  Mixing systems, i.e. flow charts or diagrams · CPC title

• B01F35/88

  by feeding the materials batchwise · CPC title

• B01F33/30

  Micromixers · CPC title