Culturing medium and method for culturing a bacterium of genus Tepidimonas

Patent metadata

Field	Value
Publication number	US-9790463-B2
Application number	US-201414229596-A
Country	US
Kind code	B2
Filing date	Mar 28, 2014
Priority date	Dec 26, 2013
Publication date	Oct 17, 2017
Grant date	Oct 17, 2017

Abstract

The disclosure provides a culturing medium for culturing a bacterium of genus Tepidimonas , including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH 4 ) 2 SO 4 ), ammonium nitrate (NH 4 NO 3 ), ammonium chloride (NH 4 Cl) and urea; phosphate; magnesium chloride (MgCl 2 ); yeast extract; and trace elements.

First claim

What is claimed is:

1. A culturing medium for culturing a bacterium of genus Tepidimonas , comprising:
a carbon source which is acetate;
a nitrogen source which is ammonium sulfate ((NH 4 ) 2 SO 4 );
a phosphate;
magnesium chloride (MgCl 2 );
a yeast extract; and
trace elements,
wherein the content of the acetate is about 0.2 g/L-5 g/L, the content of the ammonium sulfate is about 0.4 g/L-3 g/L, the content of the phosphate is about 1 g/L-12 g/L, the content of the MgCl 2 is about 0.01 g/L-0.5 g/L, the content of the yeast extract is about 0.2 g/L-5 g/L, and the content of the trace elements is about 0.01 g/L-0.3 g/L.

2. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 1 , wherein the phosphate comprises disodium hydrogen phosphate (Na 2 HPO 4 ) and dipotassium phosphate (K 2 HPO 4 ).

3. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 1 , wherein the phosphate is dipotassium phosphate (K 2 HPO 4 ).

4. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 3 , further comprising sodium bicarbonate (NaHCO 3 ).

5. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 1 , wherein the trace elements comprises zinc (Zn), manganese (Mn), boron (B), cobalt (Co), copper (Cu), nickel (Ni) and molybdenum (Mo).

6. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 1 , wherein the bacterium of genus Tepidimonas comprises Tepidimonas fonticaldi, Tepidimonas ignava, Tepidimonas aquatic or Tepidimonas taiwanesis.

7. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 1 , wherein the bacterium of genus Tepidimonas is Tepidimonas fonticaldi.

8. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 7 , wherein the Tepidimonas fonticaldi is Tepidimonas fonticaldi sp. nov. KCTC 12528BP.

9. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 1 , wherein in the culturing medium for culturing a bacterium of genus Tepidimonas , the carbon-nitrogen weight ratio of the carbon source to the nitrogen source is about 1-20.

10. The culturing medium for culturing a bacterium of genus Tepidimonas as claimed in claim 9 , wherein in the culturing medium for culturing a bacterium of genus Tepidimonas , the carbon-nitrogen weight ratio of the carbon source to the nitrogen source is 11.

11. A method for culturing a bacterium of genus Tepidimonas , comprising:
culturing a bacterium of genus Tepidimonas with the culturing medium of claim 1 .

12. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein the bacterium of genus Tepidimonas is cultured at about 30-70° C.

13. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 12 , wherein the bacterium of genus Tepidimonas is cultured at about 30-60° C.

14. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 13 , wherein the bacterium of genus Tepidimonas is cultured at about 55° C.

15. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein the bacterium of genus Tepidimonas is cultured at pH 5-pH 9.

16. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 15 , wherein the bacterium of genus Tepidimonas is cultured at pH 6-pH 9.

17. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 16 , wherein the bacterium of genus Tepidimonas is cultured at pH 7 or pH 8.

18. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein the bacterium of genus Tepidimonas is cultured under a stirring rate of about 0-500 rpm.

19. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 18 , wherein the bacterium of genus Tepidimonas is cultured under a stirring rate of about 0-200 rpm.

20. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 19 , wherein the bacterium of genus Tepidimonas is cultured under a stirring rate of about 200 rpm.

21. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein in the culturing medium for culturing a bacterium of genus Tepidimonas , the phosphate comprises disodium hydrogen phosphate (Na 2 HPO 4 ) and dipotassium phosphate (K 2 HPO 4 ).

22. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein in the culturing medium for culturing a bacterium of genus Tepidimonas , the phosphate is dipotassium phosphate (K 2 HPO 4 ).

23. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 22 , wherein the culturing medium for culturing a bacterium of genus Tepidimonas further comprises sodium bicarbonate (NaHCO 3 ).

24. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein in the culturing medium for culturing a bacterium of genus Tepidimonas , the trace elements comprises zinc (Zn), manganese (Mn), boron (B), cobalt (Co), copper (Cu), nickel (Ni) and molybdenum (Mo).

25. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein the bacterium of genus Tepidimonas comprises Tepidimonas fonticaldi, Tepidimonas ignava, Tepidimonas aquatic or Tepidimonas taiwanesis.

26. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein the bacterium of genus Tepidimonas is Tepidimonas fonticaldi.

27. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 26 , wherein the Tepidimonas fonticaldi is Tepidimonas fonticaldi sp. nov. KCTC 12528BP.

28. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 11 , wherein in the culturing medium for culturing a bacterium of genus Tepidimonas , the carbon-nitrogen weight ratio of the carbon source to the nitrogen source is about 1-20.

29. The method for culturing a bacterium of genus Tepidimonas as claimed in claim 28 , wherein in the culturing medium for culturing a bacterium of genus Tepidimonas , the carbon-nitrogen weight ratio of the carbon source to the nitrogen source is 11.

Assignees

• Ind Tech Res Inst

Inventors

• Han Yin-Lung
• Guo Tai-Rong
• Chang Jo-Shu
• Lou Yung-Chong
• Cheng Chieh-Lun
• Yu wan-ju
• Liu Chih-Hsi

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title