Amphiphilic polymer

Patent metadata

Field	Value
Publication number	US-9790287-B2
Application number	US-201514616635-A
Country	US
Kind code	B2
Filing date	Jun 24, 2015
Priority date	Aug 4, 2014
Publication date	Oct 17, 2017
Grant date	Oct 17, 2017

Abstract

The present invention relates to an amphiphilic polymer which includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution. A method of preparing an amphiphilic polymer represented by Formula 1 includes reacting a poly-gamma-glutamic acid with a reaction product of a fluorescent dye, biotin, an alkyl carboxylic acid having 1 to 10 carbon atoms or a cycloalkyl carboxylic acid having 5 to 20 carbon atoms having a carboxyl group, and dicyclo-hexylcarbodiimide (DCC) and reacting the poly-gamma-glutamic acid with DCC after reacting the poly-gamma-glutamic acid with the reaction product, and reacting the poly-gamma-glutamic acid with a hydrophilic amine and a hydrophobic amine.

First claim

What is claimed is:

1. An amphiphilic polymer represented by the following Formula 1:

where R 1 is a fluorescent dye, a biotin group, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl carbonyl group having 5 to 20 carbon atoms;
R 2 , R 3 , R 4 and R 5 have a structure independently derived from a hydroxy group, or a hydrophilic or hydrophobic amine,
repeated units of R 3 have identical or different structures, one or more of the repeated units of R 3 have a structure derived from a hydrophilic amine, one or more of the repeated units of R 3 have a structure derived from a hydrophobic amine; and n is an integer in a range of 1 to 1,000,
wherein the hydrophilic amine is an alkyl amine having 1 to 10 carbon atoms or a cycloalkyl amine having 5 to 20 carbon atoms, which is substituted with a hydroxy group or an amino group; and
the hydrophobic amine is an alkyl amine having 1 to 10 carbon atoms or a cycloalkyl amine having 5 to 20 carbon atoms.

2. The amphiphilic polymer of claim 1 , wherein the fluorescent dye is one or more types selected from the group consisting of Cy3, Cy5, fluorescein isothiocyanate (FITC), tetramethylrhodamine isothiocyanate (RITC), Alexa, 4,4,-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY), and Texas Red.

3. The amphiphilic polymer of claim 1 , wherein at least one of R 2 , R 3 , R 4 and R 5 have a structure independently derived from the hydrophilic amine.

4. The amphiphilic polymer of claim 1 , wherein at least one of R 2 , R 3 , R 4 and R 5 have a structure independently derived from the hydrophobic amine.

5. The amphiphilic polymer of claim 1 , wherein a mole ratio of a structure derived from the hydrophilic amine to a structure derived from the hydrophobic amine is in a range of 1:9 to 9:1.

6. The amphiphilic polymer of claim 1 , wherein a weight-average molecular weight is in a range of 5,000 to 50,000.

7. The amphiphilic polymer of claim 1 , wherein R 5 is the hydrophilic amine.

8. The amphiphilic polymer of claim 1 , wherein R 5 is the hydrophobic amine.

Assignees

• Univ Kookmin Ind Acad Coop Found

Inventors

• Yu Yeon-Gyu
• Sung Moon-Hee

Classifications

• C08G69/10

  Alpha-amino-carboxylic acids {(polysuccinimides C08G73/1092)} · CPC title

• C07K17/08 Primary

  the carrier being a synthetic polymer · CPC title

• C08G69/08

  derived from amino-carboxylic acids · CPC title

• C08K5/0008

  Organic ingredients according to more than one of the "one dot" groups of C08K5/01 - C08K5/59 · CPC title