Device for extracting nucleic acid from a sample

Patent metadata

Field	Value
Publication number	US-9789481-B2
Application number	US-97769707-A
Country	US
Kind code	B2
Filing date	Oct 24, 2007
Priority date	May 28, 1999
Publication date	Oct 17, 2017
Grant date	Oct 17, 2017

Abstract

The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.

First claim

What is claimed is:

1. A device for extracting nucleic acid from a sample, the sample containing cells or viruses, the device comprising a cartridge having:
a) a lysing chamber for lysing the cells or viruses to release the nucleic acid therefrom, wherein the lysing chamber contains a capture material for capturing the cells or viruses as the sample flows through the lysing chamber, and wherein the lysing chamber further contains a removal material for holding unwanted material in the lysing chamber;
b) at least one waste chamber for receiving sample fluid that has flowed through the lysing chamber;
c) a third chamber for receiving the nucleic acid, wherein the third chamber is contained within a reaction vessel and defined by a minor wall of a rigid frame and two opposing major walls of flexible sheets, and wherein the reaction vessel protrudes outwardly from the cartridge; and
d) at least one flow controller for directing the sample fluid that has flowed through the lysing chamber to flow into the at least one waste chamber and for directing the nucleic acid to flow into the third chamber.

2. The device of claim 1 , wherein the third chamber comprises a mixing chamber for mixing the nucleic acid with one or more reagents.

3. The device of claim 2 , wherein the reaction vessel further includes a reaction chamber in fluid communication with the mixing chamber for holding the nucleic acid for chemical reaction or optical detection.

4. The device of claim 2 , wherein the cartridge further includes:
i) a reaction chamber in fluid communication with the mixing chamber; and
ii) a capillary electrophoresis area in communication with the reaction chamber.

5. The device of claim 1 , wherein the third chamber comprises a reaction chamber for amplifying the nucleic acid and holding the nucleic acid for optical detection, and wherein the cartridge is in combination with an instrument having a heater for heating the reaction chamber and having at least one optical detector for detecting the nucleic acid.

6. The device of claim 1 , wherein the third chamber comprises a reaction chamber for amplifying the nucleic acid.

7. The device of claim 1 , wherein the lysing chamber is defined by at least one wall having an external surface to which an ultrasonic transducer may be coupled, and wherein the wall is sufficiently flexible to allow slight deflections in response to vibratory movements of the transducer to generate pressure waves or pressure pulses in the lysing chamber.

8. The device of claim 7 , further comprising beads in the lysing chamber for rupturing the cells or viruses.

9. The device of claim 1 , wherein the cartridge includes a sample chamber having a port for introducing a sample into the cartridge and a sample flow path extending from the sample chamber into the lysing chamber.

10. The device of claim 1 , wherein the at least one flow controller comprises at least one valve for directing the sample fluid to flow into the waste chamber via a first flow path in the cartridge and for directing the nucleic acid to flow into the third chamber via a second flow path in the cartridge.

11. The device of claim 1 , wherein the capture material comprises at least one filter.

12. The device of claim 1 , wherein the capture material comprises beads.

13. The device of claim 12 , wherein the beads are coated with antibodies or receptors for binding the cells or viruses.

14. The device of claim 1 , wherein the removal material comprises beads.

15. The device of claim 14 , wherein the beads are capable of binding proteins or peptides.

16. The device of claim 14 , wherein the beads are capable of binding salts, metal ions, or detergents in the sample.

17. The device of claim 14 , wherein the beads are ion exchange beads.

18. The device of claim 14 , wherein the beads have metal ion chelators.

19. The device of claim 1 , wherein the capture material comprises a first set of beads for capturing the cells or viruses, and wherein the removal material comprises a second set of beads for binding the unwanted material.

20. The device of claim 1 , wherein the flexible sheets are made of polypropylene, polyethylene, or polyester.

Assignees

• Petersen Kurt E
• Taylor Michael T
• Pourahmadi Farzad
• Mcmillan William A
• Chang Ronald
• Sakai Stanley H
• Ching Jesus
• Dority Douglas B
• Belgrader Phillip
• Northrup M Allen
• Cepheid

Inventors

• Petersen Kurt E
• Taylor Michael T
• Pourahmadi Farzad
• Mcmillan William A
• Chang Ronald
• Sakai Stanley H
• Ching Jesus
• Dority Douglas B
• Belgrader Phillip
• Northrup M Allen

Classifications

• Y10S435/81

  Packaged device or kit · CPC title

• B01L2300/0654

  Lenses; Optical fibres · CPC title

• Y10S436/829

  Liposomes, e.g. encapsulation · CPC title

• C12N1/066

  by physical processes · CPC title

• Y10S436/807

  Apparatus included in process claim, e.g. physical support structures · CPC title