Compositions and methods for cardiac therapy

Patent metadata

Field	Value
Publication number	US-9789224-B2
Application number	US-201113217218-A
Country	US
Kind code	B2
Filing date	Aug 24, 2011
Priority date	Aug 24, 2010
Publication date	Oct 17, 2017
Grant date	Oct 17, 2017

Abstract

Provided herein are methods and compositions for cardiac therapy. Such compositions include extracellular-matrix (ECM)-based products that can be used to support tissue repair. The compositions can be used for various purposes. In some cases, they can be introduced into a subject in order to preserve and/or repair damaged heart tissue.

First claim

What is claimed is:

1. A composition comprising digested, decellularized extracellular matrix derived from cardiac tissue, the extracellular matrix having a pore size of about 30 to 40 microns, wherein the composition is injectable through a catheter with an inner diameter of 25G or smaller, and wherein the extracellular matrix comprises glycosaminoglycan in a concentration of between about 60 to 120 μg mL of the extracellular matrix.

2. The composition of claim 1 , wherein the composition further comprises at least one digestive enzyme.

3. The composition of claim 2 , wherein the at least one digestive enzyme cleaves the matrix such that the composition gels at greater than 20° C. or wherein the at least one digestive enzyme cleaves the matrix such that the composition gels in less than 30 minutes.

4. The composition of claim 1 , wherein the digested, decellularized extracellular matrix is derived from ventricular tissue.

5. The composition of claim 1 , wherein the composition further comprises a growth factor.

6. The composition of claim 1 , wherein the composition is in a gel form within 30 minutes after delivery to in vivo tissue.

7. The composition of claim 1 , wherein the composition comprises factors that promote survival of endogenous cardiomyocytes and cardiac cells.

8. The composition of claim 1 , wherein the composition comprises: alginate and water.

9. A tissue culture device comprising: a composition comprising digested, decellularized extracellular matrix derived from cardiac tissue, the extracellular matrix having a pore size of about 30 to 40 microns; and a substrate, wherein the tissue culture device is used for in vitro tissue culture; and wherein the extracellular matrix comprises from about 10 to about 20 ug of glycosaminoglycans per mg of the extracellular matrix.

10. The device of claim 9 , wherein the substrate is a tissue culture plate.

11. The device of claim 9 , wherein the matrix is in the shape of a mold.

12. The device of claim 9 , wherein the matrix is molded to the shape of the substrate.

13. A method for repairing cardiac tissue comprising injecting in a subject the composition of claim 1 .

14. The method of claim 13 , wherein said composition is injected earlier than one month following myocardial infarction.

15. The method of claim 13 , wherein said composition degrades within three months following injection or implantation.

16. The composition of claim 1 , wherein following injection or implantation in a subject, the composition degrades within three months.

17. The composition of claim 1 , wherein the material transitions to gel from in vivo.

18. The composition of claim 1 , wherein the digested, decellularized extracellular matrix derived from cardiac tissue is enzymatically digested.

Assignees

• Christman Karen
• Singelyn Jennifer
• Dequach Jessica
• Univ California

Inventors

• Christman Karen
• Singelyn Jennifer
• Dequach Jessica

Classifications

• A61P9/04

  Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title

• A61P37/02

  Immunomodulators · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title