Polypeptides and active fragments of polypeptides having at least one esterase activity

Patent metadata

Field	Value
Publication number	US-9783793-B2
Application number	US-201214009796-A
Country	US
Kind code	B2
Filing date	Apr 11, 2012
Priority date	Apr 6, 2011
Publication date	Oct 10, 2017
Grant date	Oct 10, 2017

Abstract

The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.

First claim

The invention claimed is:

1. A polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or an active fragment of said polypeptide, further comprising at least one esterase activity, wherein the activity is a cutinase activity, a suberinase activity or a lipase activity, or any combination of said activities, and wherein the polypeptide or the fragment is active towards polyesters at pH range from 2.5 to 7.5.

2. The polypeptide or fragment of claim 1 , wherein the activity is a combination of a cutinase, a suberinase and a lipase activity.

3. The polypeptide or fragment of claim 1 , wherein the polypeptide or fragment is active towards polyesters at pH below 5.

4. The polypeptide or fragment of claim 1 , comprising at least 77 amino acids.

5. The polypeptide or fragment of claim 1 , wherein the polypeptide or fragment is derived from Sirococcus.

6. An enzyme preparation comprising a polypeptide having an amino acid sequence of SEQ ID NO: 1 or an active fragment of said polypeptide, further comprising at least one esterase activity, wherein the activity is a cutinase activity, a suberinase activity or a lipase activity, or any combination of said activities, and wherein the polypeptide or the fragment is active towards polyesters at pH range from 2.5 to 7.5.

7. A method of hydrolysis comprising the step of contacting a material containing ester bonding with the polypeptide of claim 1 under conditions suitable for hydrolysis.

8. Method of claim 7 , further comprising treating agricultural or food raw materials or by-products obtained from vegetables, fruits, grapes, berries or cereals.

9. Method of claim 7 , further comprising treating wood raw materials, pulp and paper products, or process wastes or waters, or by-products with said protein.

10. Method of claim 7 , further comprising modifying synthetic or other man-made polyester fibres or textiles with said protein.

11. Method of claim 7 , further comprising removing stickies or fat from laundry and dishes with said protein.

12. Method of trans- or interesterification comprising the step of contacting a material to be esterified with the polypeptide of claim 1 under conditions suitable for esterification.

13. The polypeptide or fragment of claim 1 , wherein the polypeptide or fragment is encoded by nucleotides 7-561 of SEQ ID NO: 3.

14. The polypeptide or fragment of claim 1 , wherein the polypeptide or fragment includes a C-terminal His-tag enabling one-step purification of the polypeptide or fragment by immobilized metal affinity chromatography.

Assignees

• Nyyssola Antti
• Kontkanen Hanna
• Hakkinen Mari
• Pihlajaniemi Ville
• Saloheimo Markku
• Buchert Johanna
• Nakari-Setala Tiina
• Teknologian Tutkimuskeskus Vtt Oy

Inventors

• Nyyssola Antti
• Kontkanen Hanna
• Hakkinen Mari
• Pihlajaniemi Ville
• Saloheimo Markku
• Buchert Johanna
• Nakari-Setala Tiina

Classifications

• D21B1/021

  by chemical means · CPC title

• C12P7/62

  Carboxylic acid esters · CPC title

• C12P7/40

  containing a carboxyl group {including Peroxycarboxylic acids} · CPC title

• C02F3/342

  characterised by the enzymes used · CPC title

• D06M16/003

  with enzymes or microorganisms · CPC title