Cell culture apparatus

Patent metadata

Field	Value
Publication number	US-9783773-B2
Application number	US-201213432658-A
Country	US
Kind code	B2
Filing date	Mar 28, 2012
Priority date	Mar 28, 2011
Publication date	Oct 10, 2017
Grant date	Oct 10, 2017

Abstract

A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

First claim

What is claimed is:

1. A cell culture apparatus comprising:
an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed;
a sampling unit configured to sample the culture solution in the cell incubator;
a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and
a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

2. The cell culture apparatus according to claim 1 , wherein the delivery flow path includes a one-way valve which does not interrupt a flow directed from the inside of the sterile space toward the outside of the sterile space and which interrupts a flow directed from the outside of the sterile space toward the inside of the sterile space.

3. The cell culture apparatus according to claim 1 , wherein the culture solution delivering section includes a buffer substance supplier configured to supply a buffer substance that is used when the sampled culture solution is delivered to the outside of the sterile space.

4. The cell culture apparatus according to claim 3 , wherein the buffer substance supplier is disposed inside the sterile space, and is connected to an end portion of the delivery flow path on a side of the inside of the sterile space.

5. The cell culture apparatus according to claim 3 , wherein the buffer substance supplier is disposed outside the sterile space, and is connected to the sterile space while maintaining a sterile condition.

6. The cell culture apparatus according to claim 1 , wherein the culture solution delivering section includes a suction pump configured to suck the culture solution that is sampled by the sampling unit, toward a portion of the delivery flow path on a side of the outside of the sterile space.

7. The cell culture apparatus according to claim 6 , wherein
the suction pump is a peristalic pump which is disposed in the delivery flow path on a side of one of the inside and the outside of the sterile space.

8. The cell culture apparatus according to claim 6 , wherein the suction pump is a negative pressure pump which is connected to an end portion of the delivery flow path on a side of the outside.

9. The cell culture apparatus according claim 1 , wherein:
the delivery flow path includes an ejection section which is disposed inside the sterile space; and
the sampling unit is configured to suck a predetermined amount of the culture solution in the cell incubator and to eject the sucked culture solution into the ejection section.

10. The cell culture apparatus according to claim 1 , further comprising a measuring device configured to perform measurement on the culture solution which is delivered to the outside of the sterile space via the delivery flow path.

11. The cell culture apparatus according to claim 10 , further comprising a calibration flow path which is connected to the buffer substance supplier while maintaining a sterile condition, and through which the inside of the sterile space and outside of the sterile space communicate with each other, wherein
the measuring device is configured to perform measurement on the buffer substance which is delivered to the outside of the sterile space via the calibration flow path.

Assignees

• Oura Mitsuhiro
• Okano Teruo
• Shimizu Tatsuya
• Kubo Hirotsugu
• Takeda Sunao
• Nihon Kohden Corp
• Tokyo Women'S Medical Univ

Inventors

• Oura Mitsuhiro
• Okano Teruo
• Shimizu Tatsuya
• Kubo Hirotsugu
• Takeda Sunao

Classifications

• C12M29/26 Primary

  Conditioning fluids entering or exiting the reaction vessel · CPC title

• C12M33/06 Primary

  for multiple inoculation or multiple collection of samples · CPC title

• C12M41/14

  Incubators; Climatic chambers (per se B01L1/00) · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M33/04

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title