Microorganisms that extracellularly secrete lipid particles encapsulating lipids

Patent metadata

Field	Value
Publication number	US-9782378-B2
Application number	US-60698306-A
Country	US
Kind code	B2
Filing date	Dec 1, 2006
Priority date	Aug 13, 1999
Publication date	Oct 10, 2017
Grant date	Oct 10, 2017

Abstract

There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid particles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.

First claim

The invention claimed is:

1. A composition comprising isolated lipid particles extracellularly secreted by a mutated Mortierella alpina , wherein the isolated lipid particles encapsulate an unsaturated fatty acid-containing lipid, wherein the isolated lipid particles comprise 20-60% sugar, 0-30% protein, and 30-80% lipid, wherein the mutated M. alpina grows into colonies covered with the lipid particles on a solid medium, or produces a cloudy culture liquid in a transparent liquid medium, and wherein the isolated lipid particles are produced by M. alpina SAM 2241 or M. alpina SAM 2242.

2. The composition of claim 1 , wherein the unsaturated fatty acid-containing lipid contains an unsaturated fatty acid having 18 or more carbons and two or more double bonds.

3. The composition of claim 1 , wherein the microorganism is cultured in a liquid medium, and wherein the isolated lipid particles are obtained from a culture liquid.

4. The composition of claim 1 , wherein the isolated lipid particles can be uniformly dispersed in water or a hydrophilic substance.

5. The composition of claim 1 , wherein the isolated lipid particles stably retain the unsaturated fatty acid-containing lipid thereby preventing oxidation of the unsaturated fatty acid.

6. The composition of claim 1 , wherein the isolated lipid particles are isolated by centrifugation and/or filtration.

7. The composition of claim 1 , wherein the isolated lipid particles have an average diameter of 0.2 to 10 μm.

8. The composition of claim 1 , wherein the unsaturated fatty acid-containing lipid comprises 50% or more triglyceride.

Assignees

• Akimoto Kengo
• Kawashima Hiroshi
• Shimizu Sakayu
• Suntory Holdings Ltd

Inventors

• Akimoto Kengo
• Kawashima Hiroshi
• Shimizu Sakayu

Classifications

• A61K8/9728

  Fungi, e.g. yeasts · CPC title

• A61K8/92

  Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof · CPC title

• A23L2/52

  Adding ingredients (adding preservatives A23B70/10) · CPC title

• A61K8/925

  of animal origin · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title