Antimicrobial delivery systems, methods of manufacture, and methods of use thereof

Patent metadata

Field	Value
Publication number	US-9781949-B2
Application number	US-201213433661-A
Country	US
Kind code	B2
Filing date	Mar 29, 2012
Priority date	Mar 31, 2011
Publication date	Oct 10, 2017
Grant date	Oct 10, 2017

Abstract

Disclosed herein is an antimicrobial delivery system comprising an electrostatic complex comprising cationic biopolymer and an anionic biopolymer, wherein the antimicrobial delivery system has antimicrobial activity in a comestible.

First claim

What is claimed is:

1. An antimicrobial delivery system for a comestible, the delivery system comprising:
an electrostatic complex comprising
a cationic biopolymer, wherein the cationic biopolymer is ε-polylysine, and
an anionic biopolymer, wherein the anionic biopolymer is gum arabic
wherein anionic biopolymer and cationic biopolymer are present in a mass ratio of anionic biopolymer to cationic biopolymer of 4 to 40,

wherein the electrostatic complex exhibits a minimum inhibitory concentration of less than 10 ppm against Zygosaccharomyces bailli or Saccharomyces cerevisiae in the comestible,
wherein a solution containing the electrostatic complex has low turbidity measured as an OD 600 of less than or equal to 0.3 as measured in a one centimeter (cm) path length optical cell, and wherein a solution containing the electrostatic complex exhibits no visible sediment or precipitates upon storage at room temperature for two weeks, when the solution includes the ε-polylysine at a concentration of 1 to 200 ppm.

2. The antimicrobial delivery system of claim 1 , wherein the comestible is a liquid.

3. The antimicrobial delivery system of claim 1 , wherein the antimicrobial activity is effective to kill or inhibit the growth of greater than or equal to about 90.0% of the microorganisms in the comestible.

4. A comestible composition comprising
a comestible component; and
an electrostatic complex comprising
a cationic biopolymer, wherein the cationic biopolymer is a ε-polylysine, and
an anionic biopolymer, wherein the anionic biopolymer is gum arabic,
wherein anionic biopolymer and cationic biopolymer are present in a mass ratio of anionic biopolymer to cationic biopolymer of about 4 to about 40,

wherein the electrostatic complex exhibits a minimum inhibitory concentration of less than 10 ppm against Zygosaccharomyces bailli or Saccharomyces cerevisiae in the comestible composition,
wherein a solution containing the electrostatic complex has low turbidity measured as an OD 600 of less than or equal to 0.3 as measured in a one centimeter (cm) path length optical cell, and wherein a solution containing the electrostatic complex exhibits no visible sediment or precipitates upon storage at room temperature for two weeks, when the solution includes the ε-polylysine at a concentration of 1 to 200 ppm.

5. The comestible composition of claim 4 , wherein no precipitates or sediments are observable in the comestible by the unaided eye after combination with the electrostatic complex.

6. A method for manufacturing an antimicrobial delivery system comprising:
combining a cationic biopolymer and an anionic biopolymer in an aqueous solution to form an electrostatic complex having antimicrobial activity in a comestible,
wherein the cationic biopolymer is a ε-polylysine, and wherein the anionic biopolymer is gum arabic, wherein anionic biopolymer and cationic biopolymer are present in a mass ratio of anionic biopolymer to cationic biopolymer of 4 to 40,
wherein the electrostatic complex exhibits a minimum inhibitory concentration of less than 10 ppm against Zygosaccharomyces bailli or Saccharomyces cerevisiae in the comestible composition,
wherein a solution containing the electrostatic complex has low turbidity measured as an OD 600 of less than or equal to 0.3 as measured in a one centimeter (cm) path length optical cell, and wherein a solution containing the electrostatic complex exhibits no visible sediment or precipitates upon storage at room temperature for two weeks, when the solution includes the ε-polylysine at a concentration of 1 to 200 ppm.

7. A method for inhibiting microbial growth in a comestible, comprising
combining an antimicrobially effective amount of an electrostatic complex comprising a cationic biopolymer and an anionic biopolymer with the comestible,
wherein the cationic biopolymer is a ε-polylysine, and wherein the anionic biopolymer is gum arabic,
wherein anionic biopolymer and cationic biopolymer are present in a mass ratio of anionic biopolymer to cationic biopolymer of 4 to 40,
wherein the electrostatic complex exhibits a minimum inhibitory concentration of less than 10 ppm against Zygosaccharomyces bailli or Saccharomyces cerevisiae in the comestible composition,
wherein a solution containing the electrostatic complex has low turbidity measured as an OD 600 of less than or equal to 0.3 as measured in a one centimeter (cm) path length optical cell, and wherein a solution containing the electrostatic complex exhibits no visible sediment or precipitates upon storage at room temperature for two weeks, when the solution includes the ε-polylysine at a concentration of 1 to 200 ppm.

8. The comestible of claim 4 , wherein the comestible is a liquid.

9. The method of claim 7 , wherein the comestible is a liquid.

Assignees

• Mcclements David Julian
• Mclandsborough Lynne
• Chang Yuhua
• Univ Massachusetts

Inventors

• Mcclements David Julian
• Mclandsborough Lynne
• Chang Yuhua

Classifications

• A01N37/46

  N-acyl derivatives · CPC title

• A01N25/10

  Macromolecular compounds · CPC title

• A23L3/3463 Primary

  Human Necessities · mapped topic

• A23L3/3526

  Human Necessities · mapped topic

• A23B2/762

  Organic compounds containing nitrogen · CPC title