Methods and systems for detecting

Patent metadata

Field	Value
Publication number	US-9778254-B2
Application number	US-66921708-A
Country	US
Kind code	B2
Filing date	Jul 18, 2008
Priority date	Jul 20, 2007
Publication date	Oct 3, 2017
Grant date	Oct 3, 2017

Abstract

A system and method wherein components of a reagent such as labeled antibodies are separated from a biologically active sensor surface by depositing the reagent on a carrier surface distinct from a sensor surface in a detection region. The present device provides a short, well-defined and controlled, pre-incubation time between the particles of interest in the sample fluid and the reagent, thereby increasing the reproducibility by providing all components in one detection region such as a detection chamber.

First claim

The invention claimed is:

1. A device for detecting the presence of an analyte in a sample fluid, the device comprising:
a detection chamber having a fixed volume, said detection chamber being delimited by two sides and at least partly delimited by distinct carrier and sensor surfaces, the carrier surface and the sensor surface being arranged to face each other and being both accessible to the sample fluid from within the detection chamber,
the carrier surface comprising a reagent or components thereof deposited only on the carrier surface,
wherein the reagent or components thereof is/are present in a dissolvable matrix, and the reagent comprises one or more probes which are coupled to magnetic or magnetizable particles,
the sensor surface being configured to sense the sample fluid within the detection chamber;
wherein the distance between the carrier surface and the sensor surface is such that the interaction time between the sample fluid and the reagent is in the range from 1 to 60 seconds, and
wherein said device further comprises:
at least a magnetic retainer for retaining the reagent or components thereof on the carrier surface; wherein the magnetic retainer comprises at least two magnetic actuators facing one to the other, and positioned on either sides of the detection chamber;
an inlet for delivering the sample fluid into the device, said inlet for the sample fluid comprising a capillary section with dimensions such that the sample fluid can be driven therein via capillary forces, and said inlet for the sample fluid being remote from the reagent or components thereof;
an outlet for removing a fluid from the device; and
a fluidic channel on both sides of the detection chamber, said fluidic channel being connected to said inlet and to said outlet such that the detection chamber presents an enlarged volume towards the inlet and/or the outlet.

2. The device according to claim 1 , wherein the volume of the detection chamber is comprised between 0.1 μl and 1 μl.

3. The device according to claim 1 , wherein the dimensions of the capillary section of the inlet for the sample fluid are 0.1 to 2 mm.

4. The device according to claim 1 , wherein the carrier surface is not co-planar with the sensor surface.

5. The device according to claim 4 , wherein a distance between the carrier surface and the sensor surface is tunable.

6. The device according to claim 4 , wherein a distance between the carrier surface and the sensor surface is between 30 μm and 500 μm.

7. The device according to claim 1 , wherein the detection chamber comprises a sloping wall that slopes towards the sensor surface.

8. The device according to claim 1 , wherein the detection chamber has a volume of 0.1 μl to 1 μl.

9. The device according to claim 1 , further comprising an actuator, other than the magnetic actuator of the magnetic retainer, for moving the reagent or components thereof in the sample fluid within the detection chamber.

10. The device according to claim 1 , wherein the reagent is comprised in a soluble material.

11. The device according to claim 1 , wherein the reagent is comprised in one or more lyophilised beads.

12. The device according to claim 1 , wherein the carrier surface is non-porous.

13. A process for manufacturing a device for detecting the presence of an analyte in a sample fluid, the process comprising:
providing a carrier surface,
depositing a reagent component in a dissolvable matrix only on to the carrier surface,
providing a sensor surface distinct from the carrier surface,
forming a detection chamber having a fixed volume, said detection chamber being delimited by two sides and at least partly delimited by the carrier surface and the sensor surface,
positioning a sensor to sense the sample fluid within the detection chamber, wherein said sensor surface is at a distance from the carrier surface such that the interaction time between the sample fluid and the reagent is in the range from 1 to 60 seconds,

and wherein said process further comprises the steps of:
forming an inlet for sample fluid at a location remote from the reagent on said carrier surface for introducing the sample fluid to the reagent within the detection chamber, said inlet for the sample fluid comprising a capillary section with dimensions such that the sample fluid can be driven therein via capillary forces, and
forming an outlet for passage of a fluid from the detection chamber that is displaced by the sample fluid, wherein the detection chamber is formed to present an enlarged volume to the inlet and/or outlet.

14. The process according to claim 13 , the process further comprising an act of drying the reagent on the carrier surface.

15. The process according to claim 13 , further comprising an act of freeze-drying the reagent.

16. The process according to claim 13 , further comprising an act of providing magnetic actuation means below and/or above the detection chamber.

17. The process according to claim 13 , further comprising an act of providing magnetic retention means above the detection chamber.

18. The process according to claim 13 , comprising an act of tuning a distance between the carrier surface and the sensor surface.

19. The process according to claim 13 , wherein forming the detection chamber comprises an act of assembling the detection chamber using the sensor surface and the carrier surface, after depositing the reagent.

20. The process according to claim 13 , wherein the process comprises an act of depositing the reagent on the carrier surface after the detection chamber has been formed.

21. A method for detecting the presence of an analyte in a sample fluid, the method comprising acts of:
providing a device comprising:
a detection chamber having a fixed volume, said detection chamber being delimited by two sides and at least partly delimited by
a carrier surface accessible to the sample fluid from within the detection chamber, the carrier surface comprising a reagent deposited only on the carrier surface in a dissolvable matrix, and
a sensor surface configured to sense the sample fluid within the detection chamber, the sensor surface being distinct from the carrier surface,

an inlet for sample fluid, said inlet for sample fluid comprising a capillary section with dimensions such that the sample fluid can be driven therein via capillary forces, and being remote from the reagent on said carrier surface, and
an outlet for passage of a fluid from the detection chamber that is displaced by the sample fluid, wherein the detection chamber is provided to present an enlarged volume to the inlet and/or outlet;

introducing the sample fluid into said detection chamber via said inlet for sample fluid,
contacting the sample fluid with the reagent deposited on said carrier surface thereby forming a fluid mixture within the detection chamber, the carrier surface being accessible to the sample fluid while the sample fluid is within the detection chamber,
contacting the fluid mixture with the sensor surface within the detection chamber, the sensor surface being distinct from the carrier surface delimiting the detection chamber and being at a distance from the carrier surface such that the interaction time between the sample fluid and the reagent is in the range from 1 to 60 seconds, and
detecting within the detection chamber through the sensor surface an interaction between the fluid mixture and the sensor surface.

22. The method according to claim 21 , wherein the method f

Assignees

• De Theije Femke Karina
• Van Der Wijk Thea
• Immink Albert Hendrik Jan
• Pelssers Eduard Gerard Marie
• Hardeman Wilhelmina Maria
• Marlin Sandra
• Jowett Gordon Thomas
• Koninklijke Philips Nv
• Concateno Uk Ltd

Inventors

• De Theije Femke Karina
• Van Der Wijk Thea
• Immink Albert Hendrik Jan
• Pelssers Eduard Gerard Marie
• Hardeman Wilhelmina Maria
• Marlin Sandra
• Jowett Gordon Thomas

Classifications

• Y10T29/49885

  with coating before or during assembling · CPC title

• G01N27/745

  for detecting magnetic beads used in biochemical assays (concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098) · CPC title

• G01N33/54366 Primary

  Apparatus specially adapted for solid-phase testing · CPC title