Device for analyzing the effect of a gaseous medium on a biological test system using an extracellular metabolization system

Patent metadata

Field	Value
Publication number	US-9777308-B2
Application number	US-201313835640-A
Country	US
Kind code	B2
Filing date	Mar 15, 2013
Priority date	Jun 29, 2007
Publication date	Oct 3, 2017
Grant date	Oct 3, 2017

Abstract

The invention relates to a method for analyzing the effect of a gaseous medium on a biological test system using an extracellular metabolization system. The method consists of the following steps: a biological test sample is cultivated on a permeable carrier, the gaseous medium is guided over the surface of the biological test system in order to form an exposition atmosphere over the biological test system, the extracellular metabolization system is added to a conservation medium and the permeable carrier is brought into contact with a conservation medium that comprises the extracellular metabolization system below the permeable carrier, in such a manner that the extracellular metabolization system only passes through the permeable carrier and that the biological test system is not submerged by the conservation medium containing the extracellular metabolization system.

First claim

What is claimed is:

1. An exposure device ( 10 ) for carrying out a method for analyzing the effect of a gaseous medium on a biological test system using an extracellular metabolization system, comprising:
at least one permeable carrier ( 30 ), on which a biological test system ( 28 ) is cultured;
compartments within the exposure device being physically separated from one another by the permeable carrier such that there is an upper compartment with an exposure atmosphere on one side of the permeable carrier and a lower compartment with a conservation medium with extracellular metabolization system on the other side of the permeable carrier;
wherein the permeable carrier is designed in such a way as to enable the accommodation of the biological test system and to enable the separation of the exposure atmosphere from the conservation medium with extracellular metabolization system; wherein
the permeable carrier ( 30 ) is disposed on the bottom ( 36 ) of a culture vessel ( 32 ) or forms this bottom ( 36 ), wherein the culture vessel ( 32 ) is disposed in an accommodation ( 26 ) of the exposure device ( 10 ), and a seal ( 38 ) is disposed between the outside wall of the culture vessel ( 32 ) and the wall of the accommodation ( 26 ), wherein
at least one feed pipe, which can route the gaseous medium over the surface of the biological test system to form the exposure atmosphere above the biological test system ( 28 ) within the upper compartment;
a delivery pipe ( 24 , 40 ), which routes the conservation medium with extracellular metabolization system into the lower compartment, wherein
the conservation medium with extracellular metabolization system is in direct contact with the underside of the permeable carrier ( 30 ), wherein
the delivery pipe ( 24 , 40 ) and the lower compartment are sealed off from the feed pipe ( 52 ) and the upper compartment such that
the conservation medium with extracellular metabolization system can only be forced through the permeable carrier ( 30 ) in a defined manner by means of a prescribed pressure, wherein the prescribed pressure can be set hydrostatically, or via at least one pump or some other pressure-generating means, in such a way that the conservation medium with extracellular metabolization system passes through the permeable carrier ( 30 ), but the biological test system ( 28 ) is not flooded by the conservation medium with extracellular metabolization system.

2. The exposure device according to claim 1 , wherein a discharge pipe is provided, with which the exposure atmosphere can be removed from the exposure device after residing therein for a predetermined period, wherein the discharge pipe is connected with the upper compartment.

3. The exposure device according to claim 2 , wherein means to generate an underpressure in the discharge pipe is provided such that the gaseous medium can be relayed in controlled fashion through the exposure device.

4. The exposure device according to claim 1 , wherein means to generate an overpressure in the feed pipe is provided such that the gaseous medium can be relayed in controlled fashion through the exposure device.

5. The exposure device according to claim 1 , wherein a removal pipe is provided with which the conservation medium with extracellular metabolization system can be removed from the exposure device after residing therein for a prescribed retention period, wherein the removal pipe is connected with the lower compartment.

6. The exposure device according to claim 5 , wherein the delivery and removal pipe comprising at least one pump such that the conservation medium with extracellular metabolization system is routed through the delivery pipe, the lower compartment and the removal pipe in a continuous or pulsating flow.

7. The exposure device according to claim 1 , wherein at least one heating device is provided, with which the temperature of the exposure device can be entirely or partially controlled.

8. The exposure device according to claim 1 , wherein the biological test system is either a eukaryote culture or a prokaryote culture.

9. The exposure device according to claim 1 , wherein it is combined with a cell based sensor array.

Assignees

• Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E V

Inventors

• Knebel Jan
• Ritter Detlef

Classifications

• C12M41/46

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• G01N33/5014

  for testing toxicity · CPC title

• G01N33/5008

  for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics · CPC title

• C12M25/04 Primary

  in combination with well or multiwell plates, i.e. culture inserts · CPC title

• C12Q1/02 Primary

  involving viable microorganisms · CPC title