Recombinant yeast transformant and process for preparing immunoglobulin fc fragment employing the same
US-2015361437-A1 · Dec 17, 2015 · US
US9777280B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9777280-B2 |
| Application number | US-201615060036-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 3, 2016 |
| Priority date | Nov 22, 2010 |
| Publication date | Oct 3, 2017 |
| Grant date | Oct 3, 2017 |
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The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP.
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What is claimed is: 1. A genetically modified yeast cell selected from the group consisting of I. orientalis, C. lambica , and S. bulderi , the genetically modified yeast cell comprising an active 3-HP fermentation pathway, wherein the cell comprises an exogenous BAAT or gabT gene encoding an enzymatically active polypeptide with at least 85% sequence identity to an amino acid sequence selected from SEQ ID NOs: 20, 21, and 24. 2. The genetically modified yeast cell of claim 1 , wherein the BAAT or gabT gene encodes a polypeptide having at least 90% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21, and 24. 3. The genetically modified yeast cell of claim 1 , wherein the BAAT or gabT gene encodes a polypeptide having at least 95% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21, and 24. 4. The genetically modified yeast cell of claim 1 , wherein the BAAT or gabT gene encodes a polypeptide having at least 97% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21, and 24. 5. The genetically modified yeast cell of claim 1 , wherein the BAAT or gabT gene encodes a polypeptide having at least 98% sequence identity to the amino acid sequence selected from SEQ ID Nos: 20, 21, and 24.
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