Human placental collagen compositions, and methods of making and using the same

Patent metadata

Field	Value
Publication number	US-9775886-B2
Application number	US-201213610375-A
Country	US
Kind code	B2
Filing date	Sep 11, 2012
Priority date	Oct 6, 2006
Publication date	Oct 3, 2017
Grant date	Oct 3, 2017

Abstract

The present invention provides compositions comprising human placental telopeptide collagen, methods of preparing the compositions, methods of their use and kits comprising the compositions. The compositions, kits and methods are useful, for example, for augmenting or replacing tissue of a mammal.

First claim

What is claimed is:

1. A composition comprising placental extracellular matrix, wherein said composition comprises at least 80% collagen by dry weight, wherein said collagen comprises (i) between 74% and 90% Type I collagen, between 4% to 6% Type III collagen, and between 2% to 15% type IV collagen, (ii) no detectable amounts of fibronectin or laminin, and (iii) additional extracellular matrix components selected from the following groups:
(a) 4.7% elastin by dry weight and 0.28% glycosaminoglycans by dry weight;
(b) 3.2% elastin by dry weight and 0.34% glycosaminoglycans by dry weight;
(c) 4.4% elastin by dry weight and 0.38% glycosaminoglycans by dry weight; and
(d) 3.5% elastin by dry weight and 0.59% glycosaminoglycans by dry weight.

2. The composition of claim 1 , wherein said placental collagen is human placental collagen.

3. The composition of claim 1 , wherein said composition is shaped as a sheet.

4. The composition of claim 1 , wherein said composition is shaped to fit to a site of a wound or injury.

5. The composition of claim 1 , wherein said composition is formulated as a gel, a cream, a paste, a spray, an aerosol, or a solution.

6. The composition of claim 1 , wherein said composition comprises a pharmacologically active agent.

7. The composition of claim 6 , wherein said pharmacologically active agent comprises platelet-derived growth factor, insulin-like growth factor, epidermal growth factor, transforming growth factor beta, an angiogenesis factor, an antibiotic, an antifungal agent, a spermicidal agent, a hormone, an enzyme, or an enzyme inhibitor.

8. The composition of claim 1 , wherein said composition is prepared by performing the following steps, in order:
a. macerating placental tissue;
b. suspending the tissue in an osmotic shock solution;
c. suspending the tissue in a solution that comprises detergent; and
d. washing the tissue.

9. The composition of claim 1 , wherein the composition further comprises placental stem cells that are allogeneic to the subject.

10. The composition of claim 1 , wherein the composition further comprises placental stem cells that are autologous to the subject.

11. The composition of claim 8 , wherein the detergent comprises deoxycholate.

12. A method of treating a wound or burn of a subject comprising contacting the wound or burn with the composition of claim 1 .

13. The method of claim 12 , wherein said composition is used as a wound filler.

14. The method of claim 12 , wherein said composition is applied directly to the site of the wound or burn.

15. A method of augmenting the skin of a subject in an area of the subject in need of augmenting, wherein said method comprises administering the composition of claim 1 to said area.

16. The method of claim 15 , wherein said composition is administered by injection.

17. The method of claim 15 , wherein said composition is administered to augment skin that requires augmentation as a result aging, trauma, disease, sickness, environmental factors, weight loss, or child birth.

18. The method of claim 15 , wherein said composition is administered to augment skin that comprises wrinkles, frown lines, worry lines, crow's feet, marionette lines, stretch marks, or scars.

Assignees

• Bhatia Mohit
• Lugo Chris
• Ye Qian
• Edinger James W
• Celularity Inc

Inventors

• Bhatia Mohit
• Lugo Chris
• Ye Qian
• Edinger James W

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P17/02

  for treating wounds, ulcers, burns, scars, keloids, or the like · CPC title

• G02B6/2746

  comprising non-reciprocal devices, e.g. isolators, FRM, circulators, quasi-isolators (magneto-optic non-reciprocal devices G02F1/093) · CPC title

• G02B6/1228

  Tapered waveguides, e.g. integrated spot-size transformers (for coupling with fibres G02B6/305) · CPC title

• G02B6/12004

  Combinations of two or more optical elements · CPC title