Polypeptides having protease activity

Patent metadata

Field	Value
Publication number	US-9771570-B2
Application number	US-201314423546-A
Country	US
Kind code	B2
Filing date	Sep 5, 2013
Priority date	Sep 5, 2012
Publication date	Sep 26, 2017
Grant date	Sep 26, 2017

Abstract

The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed.

First claim

What is claimed is:

1. A method of producing an S53 protease, comprising cultivating a recombinant host cell comprising an exogenous polynucleotide encoding the S53 protease under conditions conducive for production of the S53 protease, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the S53 protease in the recombinant host cell and wherein the amino acid sequence of the S53 protease polypeptide is at least 90% identical to the polypeptide of SEQ ID NO: 5.

2. The method of claim 1 , wherein the amino acid sequence of the S53 protease polypeptide is at least 95% identical to the polypeptide of SEQ ID NO: 5.

3. The method of claim 1 , wherein the amino acid sequence of the S53 protease polypeptide is at least 96% identical to the polypeptide of SEQ ID NO: 5.

4. The method of claim 1 , wherein the amino acid sequence of the S53 protease polypeptide is at least 97% identical to the polypeptide of SEQ ID NO: 5.

5. The method of claim 1 , wherein the amino acid sequence of the S53 protease polypeptide is at least 98% identical to the polypeptide of SEQ ID NO: 5.

6. The method of claim 1 , wherein the amino acid sequence of the S53 protease polypeptide is at least 99% identical to the polypeptide of SEQ ID NO: 5.

7. The method of claim 1 , wherein the amino acid sequence of the S53 protease is the polypeptide of SEQ ID NO: 5.

8. The method of claim 1 , wherein the amino acid sequence of the S53 protease is a fragment of the polypeptide of SEQ ID NO: 5.

9. The method of claim 1 , further comprising recovering the S53 protease.

10. The method of claim 1 , wherein the recombinant host cell is a recombinant fungal host cell.

11. The method of claim 10 , wherein the recombinant fungal host cell is a recombinant filamentous fungal host cell.

12. The method of claim 11 , wherein the recombinant filamentous fungal host cell is selected from the group consisting of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes , and Trichoderma recombinant host cells.

13. The method of claim 12 , wherein the recombinant filamentous fungal host cell is selected from the group consisting of Aspergillus awamori, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Chrysosporium inops, Chrysosporium keratinophilum, Chrysosporium lucknowense, Chrysosporium merdarium, Chrysosporium pannicola, Chrysosporium queenslandicum, Chrysosporium tropicum, Chrysosporium zonatum, Coprinus cinereus, Coriolus hirsutus, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium suiphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Humicola insolens, Humicola lanuginose, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Penicillium purpurogenum, Phanerochaete chrysosporium, Phlebia radiata, Pleurotus eryngii, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei , and Trichoderma viride recombinant host cells.

14. The method of claim 2 , further comprising recovering the S53 protease.

15. The method of claim 2 , wherein the recombinant host cell is a recombinant fungal host cell.

16. The method of claim 15 , wherein the recombinant fungal host cell is a recombinant filamentous fungal host cell.

17. The method of claim 16 , wherein the recombinant filamentous fungal host cell is selected from the group consisting of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes , and Trichoderma recombinant host cells.

18. The method of claim 17 , wherein the recombinant filamentous fungal host cell is selected from the group consisting of Aspergillus awamori, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Chrysosporium inops, Chrysosporium keratinophilum, Chrysosporium lucknowense, Chrysosporium merdarium, Chrysosporium pannicola, Chrysosporium queenslandicum, Chrysosporium tropicum, Chrysosporium zonatum, Coprinus cinereus, Coriolus hirsutus, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium suiphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Humicola insolens, Humicola lanuginose, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Penicillium purpurogenum, Phanerochaete chrysosporium, Phlebia radiata, Pleurotus eryngii, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei , and Trichoderma viride recombinant host cells.

Assignees

• Novozymes As

Inventors

• Stringer Mary Ann
• Hoff Tine
• Oestergaard Peter Rahbek
• Pontoppidan Katrine

Classifications

• A23K20/26

  Compounds containing phosphorus · CPC title

• C12N9/485 Primary

  Exopeptidases (3.4.11-3.4.19) · CPC title

• A23K50/75

  for poultry · CPC title

• A23K50/20

  for horses · CPC title

• A23K20/189

  Enzymes · CPC title