Methods of reprogramming renal cells

Patent metadata

Field	Value
Publication number	US-9770469-B2
Application number	US-201314027256-A
Country	US
Kind code	B2
Filing date	Sep 16, 2013
Priority date	Feb 26, 2009
Publication date	Sep 26, 2017
Grant date	Sep 26, 2017

Abstract

A method of reprogramming a differentiated renal cell towards a progenitor phenotype is disclosed. The method comprises up-regulating in the differentiated renal cell an expression of at least one pluripotency associated gene and/or at least one renal stem cell associated gene, thereby reprogramming the differentiated renal cell towards a progenitor phenotype. Cell populations generated thereby and uses thereof are also disclosed.

First claim

What is claimed is:

1. A method of reprogramming a differentiated adult epithelial renal cell to a cell having a progenitor phenotype, the method comprising transfecting the differentiated renal cell with a nucleic acid construct comprising a nucleic acid sequence encoding Oct4, thereby reprogramming the differentiated adult epithelial renal cell to a cell having a progenitor phenotype, and wherein said progenitor phenotype comprises Six2 and/or Osr1 expression and cell clonogenicity.

2. The method of claim 1 , further comprising isolating the cell having the progenitor phenotype.

3. The method of claim 1 , further comprising contacting the differentiated adult epithelial renal cell with at least one chromatin modifying agent.

4. The method of claim 1 , wherein said differentiated adult epithelial renal cell is a human differentiated adult epithelial renal cell.

5. The method of claim 3 , wherein said chromatin modifying agent is selected from the group consisting of valporic acid, 5-aza-2′-deoxycytidine and TSA.

6. A method of reprogramming a differentiated adult epithelial renal cell to a cell having a renal progenitor phenotype, the method comprising transfecting the differentiated adult epithelial renal cell with a nucleic acid construct comprising a nucleic acid sequence encoding at least one polypeptide which comprises Six2, thereby reprogramming the differentiated renal cell to a cell having a renal progenitor phenotype.

7. The method of claim 6 , wherein said at least one polypeptide comprises Osr1 and Six2.

Assignees

• Tel Hashomer Medical Res Infrastructure & Services Ltd

Inventors

• Dekel Benjamin
• Harari-Steinberg Orit

Classifications

• C12N2500/90

  Serum-free medium, which may still contain naturally-sourced components · CPC title

• C12N2506/25

  from renal cells, from cells of the urinary tract · CPC title

• C12N15/85

  for animal cells · CPC title

• C12N2501/065

  Modulators of histone acetylation · CPC title

• C12N5/0696

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title