Amphiphilic dendron-coils, micelles thereof and uses

Patent metadata

Field	Value
Publication number	US-9770413-B2
Application number	US-201514968867-A
Country	US
Kind code	B2
Filing date	Dec 14, 2015
Priority date	Feb 23, 2011
Publication date	Sep 26, 2017
Grant date	Sep 26, 2017

Abstract

The invention generally relates to the fields of drug delivery and cell capture. In particular, the invention relates to amphiphilic dendron-coils, micelles thereof and their use for drug delivery vehicles and/or cell capture.

First claim

We claim:

1. A method of capturing a cell from a sample comprising the steps of:
exposing the sample to an immobilized amphiphilic dendron-coil comprising a non-peptidyl, hydrophobic core-forming block, a polyester dendron and a poly(ethylene) glycol (PEG) moiety with a ligand conjugated to the PEG, wherein the non-peptidyl, hydrophobic core-forming block comprises a linear hydrophobic polymer and wherein the ligand binds to the cell; and
capturing the cell by binding of the ligand to the cell.

2. The method of claim 1 wherein the non-peptidyl, hydrophobic core-forming block comprises polycaprolactone (PCL), poly(lactic acid) (PLA), poly(glycolic acid) (PGA) or poly(lactic-co-glycolic acid) (PLGA); wherein the polyester dendron is a generation 3 to generation 5 polyester dendron with either an acetylene or carboxylate core; and wherein the PEG moiety is a methoxy PEG (mPEG) moiety, amine-terminated PEG (PEG-NH 2 ) moiety, acetylated PEG (PEG-Ac) moiety, carboxylated PEG (PEG-COOH) moiety, thiol-terminated PEG (PEG-SH) moiety, N-hydroxysuccinimide-actived PEG (PEG-NHS) moiety, NH 2 -PEG-NH 2 moiety or NH 2 -PEG-COOH moiety.

3. The method of claim 2 wherein the non-peptidyl, hydrophobic core-forming block has a molecular weight from about 0.5 kDa to about 20 kDa.

4. The method of claim 1 wherein the polyester dendron is a generation 3 (G3) dendron.

5. The method of claim 1 wherein the polyester dendron is a generation 4 (G4) dendron.

6. The method of claim 1 wherein the polyester dendron is a generation 5 (G5) dendron.

7. The method of claim 4 wherein the polyester dendron is a generation 3 polyester-8-hydroxyl-1-acetylene bis-MPA dendron.

8. The method of claim 1 wherein the PEG moiety is a methoxy PEG (mPEG) moiety, amine-terminated PEG (PEG-NH 2 ) moiety, acetylated PEG (PEG-Ac) moiety, carboxylated PEG (PEG-COOH) moiety, thiol-terminated PEG (PEG-SH) moiety, N-hydroxysuccinimide-actived PEG (PEG-NHS) moiety, NH 2 -PEG-NH 2 moiety or NH 2 -PEG-COOH moiety.

9. The method of claim 7 wherein the PEG moiety has a molecular weight from about 0.2 kDa to about 5 kDa.

Assignees

• Univ Illinois

Inventors

• Hong Seungpyo
• Bae Jin Woo

Classifications

• C08G63/66

  Polyesters containing oxygen in the form of ether groups (C08G63/42, C08G63/58 take precedence) · CPC title

• A61K9/1075 Primary

  Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers (A61K9/0026 takes precedence) · CPC title

• C12Q1/02

  involving viable microorganisms · CPC title

• C08G83/003

  Dendrimers · CPC title

• C08G83/004

  After treatment of dendrimers · CPC title