Compositions and methods for treating pests
US-2015373994-A1 · Dec 31, 2015 · US
US9770033B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9770033-B2 |
| Application number | US-201615154069-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 13, 2016 |
| Priority date | May 14, 2015 |
| Publication date | Sep 26, 2017 |
| Grant date | Sep 26, 2017 |
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Disclosed are genetically modified nudiviruses capable of being sexually transmitted by an insect useful for controlling pest populations. The genetically modified nudiviruses are capable of causing sterility in a target population of insects. Also disclosed are insects infected with the disclosed genetically modified nudiviruses, methods of making the genetically modified nudiviruses, and methods of using the genetically modified nudiviruses to control an insect pest population.
Opening claim text (preview).
What is claimed is: 1. A genetically modified Helicoverpa zea nudivirus 2 (HzNV-2) virus, having a mutation in a region selected from Open Reading Frame 90 (ORF90), Open Reading Frame 92 (ORF92), Persistance-associated Gene (pag1), or a combination thereof, to disrupt expression of the gene product encoded by said mutant region, wherein said mutation increases the rate of an agonadal phenotype in a progeny of a Helicoverpa zea ( H. zea ) moth infected with said genetically modified HzNV-2 as compared to a progeny of a H. zea moth infected with a wild-type HzNV-2 virus. 2. The genetically modified nudivirus of claim 1 , wherein said genetically modified HzNV-2 virus is achieved via chemical mutagenesis. 3. The genetically modified nudivirus of claim 1 , wherein said genetically modified HzNV-2 virus is achieved via recombinant DNA technology. 4. The genetically modified nudivirus of claim 1 , wherein said genetically modified HzNV-2 virus is achieved via gene editing technology. 5. An insect infected with the virus of claim 1 . 6. The insect of claim 5 , wherein said insect is H. zea. 7. A method of protecting a crop susceptible to H. zea moth crop damage, comprising the step of introducing an H. zea moth infected with the genetically modified nudivirus of claim 1 into said crop.
Animals; Substances produced thereby or obtained therefrom · CPC title
Uses of virus other than therapeutic or vaccine, e.g. disinfectant · CPC title
Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title
Viruses as such, e.g. new isolates, mutants or their genomic sequences · CPC title
Demonstrated in vivo effect · CPC title
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