Method for automatic behavioral phenotyping

Patent metadata

Field	Value
Publication number	US-9767230-B2
Application number	US-201214236662-A
Country	US
Kind code	B2
Filing date	Aug 3, 2012
Priority date	Aug 3, 2011
Publication date	Sep 19, 2017
Grant date	Sep 19, 2017

Abstract

A method of identifying and classifying social complex behaviors among a group of model organisms, comprising implanting at least one RFID transponder in each model organism in said group of model organisms; enclosing said group of model organisms in a monitored space divided into RFID monitored segments; RFID tracking a position of each model organism by reading said at least one RFID transponder in each model organism over a period of time; capturing a sequence of images of each model organism over said period of time; and calculating at least one spatiotemporal model of each model organism based on time synchronization of said RFID tracked position of said model organism with said sequence of images.

First claim

What is claimed is:

1. A method for identifying and classifying complex social behaviors among a group of model organisms, comprising:
automatically tracking the momentary location and identification of one or more model organisms in a group of 3 or more organisms living in an enclosure comprising a large environment with an area matching a natural habitat size for said group over a period of time to provide data,
wherein said tracking includes the use of radio frequency identification (RFID) technology using detectors with a resolution of better than about 5 cm;
processing said location and identification data to provide one or more parameters of movement including trajectories for said one or more model organisms;
processing said data to identify a social relationship between at least two model organisms; and
evaluating said social relationship to identify a behavioral phenotype in at least one of said model organisms.

2. A method according to claim 1 wherein processing said data includes automatically calculating locomotion information for at least two of said model organisms.

3. A method according to claim 1 , comprising automatically quantifying said social relationship.

4. A method according to claim 1 wherein processing said data includes generating at least one behavioral parameter based on a plurality of other behavioral parameters.

5. A method according to claim 1 , wherein processing said data includes calculating for one or more of said model organisms any one of, or any combination of, activity/resting time, circadian activity pattern, sleeping/resting time, mean velocity, daily travel distance, time chasing, and time being chased.

6. A method according to claim 1 wherein said automatic tracking includes use of video imaging having positional accuracy in the range of about 0.1 cm to about 3 cm.

7. A method according to claim 1 wherein said detectors have a spatial resolution of better than 5 cm.

8. A method according to claim 7 , wherein said detection includes video imaging and said tracking comprises fusing a succession of video images and RFID information.

9. A method according to claim 1 comprising classifying said one or more model organisms based on said behavioral phenotype.

10. A method of identifying and classifying complex social behaviors among a group of model organisms, each having a permanently affixed or implanted RFID transponder, comprising:
enclosing said group of model organisms in a monitored space divided into RFID monitored segments;
RFID tracking a location, at a spatial resolution of 10 cm or better, of each model organism by reading the RFID transponder in each model organism over a period of time,
wherein said reading is performed using a plurality of detectors distributed in and around said enclosure;
capturing a sequence of images of one or more model organisms over said period of time; and
calculating at least one spatiotemporal model of each model organism based on time synchronization of said RFID identifying information of said model organism with said sequence of images.

11. A method according to claim 10 wherein said spatiotemporal model includes calculating a best path for each model organism by extrapolating between imaged positions of each model organism in at least a portion of said sequence of images and predicted positions.

12. A method according to claim 10 wherein said RFID tracking includes reading at least one transponder as each model organism with a transponder moves across segments.

13. A method according to claim 10 , wherein said calculating comprises fusing video data and RFID data.

14. A method according to claim 13 , wherein said fusing comprises using RFID data to assist in clustering video data or using video data to improve a trajectory provided by RFID identification data.

15. A method according to claim 10 , wherein capturing a sequence of images comprises generating a reference image based on a plurality of images acquired while said organisms are being imaged.

16. A system, comprising:
an enclosure for accommodating a group of at least 3 model organisms, each model organism having at least one RFID transponder;
a plurality of RFID readers distributed in said enclosure such that their RF coverage divides said enclosed space into RFID monitored segments,
an imaging system, providing resolution of between about 0.1 and about 3 cm for capturing a sequence of images of said enclosed space;
a processor configured to provide one or both of (a) at least one spatiotemporal model for one or more model organisms in said group (b) trajectories of one or more of said model organisms based on combined RFID data and said sequence of imaging data for said model organisms.

17. A system according to claim 16 , wherein said processor is configured to provide both of said (a) and (b).

18. A system according to claim 16 , wherein said processor is configured to automatically classify a phenotype or quantify a behavior of said animal.

19. A system according to claim 16 , wherein said processor is configured to automatically extract social behavior for a plurality of said model organisms.

20. A system according to claim 16 , wherein said processor is configured to collect information correlated with a treatment of said model organisms.

21. A system according to claim 16 , configured to identify one or more trigger conditions and to store data, collect data and/or control an actuator in the system, in response thereto.

22. A system according to claim 16 , wherein said enclosed space comprises a large environment of an area matching a natural habitat size for said group.

23. A system according to claim 16 , configured to track a vertical component of position in at least part of said enclosure.

24. A method according to claim 1 , wherein said tracking comprises fusing video tracking and RFID identification information.

25. A method according to claim 1 , wherein said trajectory tracking is performed by a plurality of detection modalities using detectors distributed in and around said enclosure.

26. A method according to claim 1 , further including comparing the trajectories of two of said model organisms.

27. A method according to claim 1 , wherein said trajectories are substantially continuous.

28. A method according to claim 27 , wherein providing said trajectories includes calculating a continuous best path for each model organism from a sequence of location data for said model organisms.

29. A method according to claim 6 , wherein the video imaging has a time accuracy of between 0.01 and 1 second.

30. A method according to claim 10 , wherein processing said data to identify a social relationship between at least two model organisms includes generating trajectories for said model organisms.

31. A method according to claim 30 , wherein said trajectories are generated by calculating a continuous path from a sequence of said images of said model organisms.

32. A method according to claim 31 , wherein said continuous path is a calculated best path for each model organism.

33. A method according to claim 10 , wherein said reading includes using a plurality of detectors uniformly distributed at the bottom of said enclosure.

34. A method according to claim 10 , further includin

Assignees

• Kimchi Tali
• Weissbrod Aharon
• Vasserman Genadiy
• Yeda Res & Dev

Inventors

• Kimchi Tali
• Weissbrod Aharon
• Vasserman Genadiy

Classifications

• G06F30/20

  Design optimisation, verification or simulation (optimisation, verification or simulation of circuit designs G06F30/30) · CPC title

• G06F18/24323

  Tree-organised classifiers · CPC title

• G01S3/7864

  T.V. type tracking systems · CPC title

• A01K29/005 Primary

  Monitoring or measuring activity · CPC title

• G01S3/14

  Systems for determining direction or deviation from predetermined direction {(aerial arrangements for changing or varying the orientation or the shape of the directional pattern H01Q3/00; combinations of different interacting aerial units for giving a desired directional characteristic H01Q21/29; aerials or aerial systems providing at least two radiation patterns H01Q25/00)} · CPC title