Peptide with gold binding and EGFR receptor affinity and same attached to gold nanostructure

Patent metadata

Field	Value
Publication number	US-9766252-B2
Application number	US-201314386269-A
Country	US
Kind code	B2
Filing date	Mar 13, 2013
Priority date	Mar 22, 2012
Publication date	Sep 19, 2017
Grant date	Sep 19, 2017

Abstract

An embodiment of the invention is a peptide comprising four domains, wherein domain I consists of thioctyl or monocytl, domain II consists of 2 to 3 positively charged amino acids selected from the group consisting of lysine and arginine, domain III consists of a dimeric ethylene unit; and domain IV comprises the peptide with SEQ ID No. 1 or a sequence having at least 90% identity to SEQ ID No. I. The peptide is preferably attached to a gold nanostructure, preferably a gold nanorod to provide an EFGR kit. An EFGR detection kit of the invention employs a gold nanostructure attached to a peptide sequence, the peptide sequence includes a binding sequence with an affinity toward EGFR, a ligand bound to gold atoms of the nanorod, a positively charged amino acid that maintains activity of the binding sequence, and a unit that increases hydrophilicity of the peptide sequence.

First claim

The invention claimed is:

1. A peptide comprising four domains, wherein domain I consists of thioctyl or monothiol, domain II consists of 2 to 3 positively charged amino acids selected from the group consisting of lysine and arginine, domain III consists of a dimeric ethylene unit; and domain IV comprises the peptide with SEQ ID No. 1.

2. The peptide of claim 1 , wherein domain I consists of thioctyl.

3. The peptide of claim 2 , wherein domain II consists of 2 molecules of lysine.

4. The peptide of claim 1 , attached to a gold nanostructure.

5. The peptide of claim 4 , wherein the gold nanostructure comprises a gold nanorod.

6. An epidermal growth factor receptor (EGFR) detection kit comprising a solution of peptides attached to the gold nanorod according to claim 5 .

7. An epidermal growth factor receptor (EGFR) detection kit comprising a solution of peptides attached to gold nanorods according to claim 4 .

8. An epidermal growth factor receptor (EGFR) detection kit comprising: a gold nanostructure attached to a peptide comprising four domains, wherein domain I consists of thioctyl or monothiol, domain II consists of 2 to 3 positively charged amino acids selected from the group consisting of lysine and arginine, domain III consists of a dimeric ethylene unit and domain IV comprises the peptide with SEQ ID No. 1.

9. The EGFR detection kit of claim 8 , wherein the positively charged amino acids consists of 2 or 3 molecules of lysine.

10. An epidermal growth factor receptor (EGFR) detection kit comprising a solution of peptides attached to the gold nanostructure according to claim 4 .

Assignees

• Univ Missouri

Inventors

• Kannan Raghuraman
• Arthur Gerald
• Caldwell Jr Charles W
• Caldwell Charles
• Chanda Mripen
• Zambre Ajit
• Ramachandran Mythili

Classifications

• C07K17/14

  Peptides being immobilised on, or in, an inorganic carrier · CPC title

• G01N33/6872 Primary

  Intracellular protein regulatory factors and their receptors, e.g. including ion channels · CPC title

• G01N33/54346

  Nanoparticles · CPC title

• G01N2333/485

  Epidermal growth factor [EGF] (urogastrone) · CPC title

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title