Modulators and methods of use

Patent metadata

Field	Value
Publication number	US-9765136-B2
Application number	US-201615072930-A
Country	US
Kind code	B2
Filing date	Mar 17, 2016
Priority date	Dec 8, 2010
Publication date	Sep 19, 2017
Grant date	Sep 19, 2017

Abstract

Novel modulators, including antibodies and derivatives thereof, and methods of such modulators to treat hyperproliferative disorders are provided.

First claim

The invention claimed is:

1. A nucleic acid encoding a heavy chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 113, 121, 137, 157, or 161, or encoding a light chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 115, 123, 139, 159, or 163.

2. The nucleic acid of claim 1 , which encodes a heavy chain variable region comprising:
(a) an amino acid sequence set forth as SEQ ID NO: 113;
(b) an amino acid sequence set forth as SEQ ID NO: 121;
(c) an amino acid sequence set forth as SEQ ID NO: 137;
(d) an amino acid sequence set forth as SEQ ID NO: 157; or
(e) an amino acid sequence set forth as SEQ ID NO: 161.

3. The nucleic acid of claim 2 , wherein
(a) comprises a nucleotide sequence set forth as SEQ ID NO: 112;
(b) comprises a nucleotide sequence set forth as SEQ ID NO: 120;
(c) comprises a nucleotide sequence set forth as SEQ ID NO: 136;
(d) comprises a nucleotide sequence set forth as SEQ ID NO: 156; and
(e) comprises a nucleotide sequence set forth as SEQ ID NO: 160.

4. The nucleic acid of claim 1 , which encodes a light chain variable region comprising:
(a) an amino acid sequence set forth as SEQ ID NO: 115;
(b) an amino acid sequence set forth as SEQ ID NO: 123;
(c) an amino acid sequence set forth as SEQ ID NO: 139;
(d) an amino acid sequence set forth as SEQ ID NO: 159; or
(e) an amino acid sequence set forth as SEQ ID NO: 163.

5. The nucleic acid of claim 4 , wherein
(a) comprises a nucleotide sequence set forth as SEQ ID NO: 114;
(b) comprises a nucleotide sequence set forth as SEQ ID NO: 122;
(c) comprises a nucleotide sequence set forth as SEQ ID NO: 138;
(d) comprises a nucleotide sequence set forth as SEQ ID NO: 158; and
(e) comprises a nucleotide sequence set forth as SEQ ID NO: 162.

6. A vector comprising at least one of a nucleic acid encoding a heavy chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 113, 121, 137, 157, or 161 and a nucleic acid encoding light chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 115, 123, 139, 159, or 163.

7. The vector of claim 6 , comprising at least one of:
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 113 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 115;
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 121 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 123;
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 137 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 139;
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 157 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 159; or
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 161 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 163.

8. The vector of claim 6 , further comprising a promoter that controls expression of the nucleic acid.

9. The vector of claim 7 , further comprising a promoter that controls expression of the nucleic acid of (a) or the nucleic acid of (b).

10. The vector of claim 7 , comprising the nucleic acid of (a) and the nucleic acid of (b).

11. The vector of claim 10 , further comprising a promoter that controls expression of the nucleic acid of (a) and the nucleic acid of (b).

12. The vector of claim 10 , further comprising a first promoter that controls expression of the nucleic acid of (a) and a second promoter that controls expression of the nucleic acid of (b).

13. A host cell comprising at least one of a nucleic acid encoding a heavy chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 113, 121, 137, 157, or 161 and a nucleic acid encoding a light chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 115, 123, 139, 159, or 163.

14. The host cell of claim 13 , comprising at least one of:
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 113 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 115;
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 121 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 123;
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 137 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 139;
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 157 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 159; or
(a) a nucleic acid encoding a heavy chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 161 and (b) a nucleic acid encoding a light chain variable region comprising the amino acid sequence set forth as SEQ ID NO: 163.

15. The host cell of claim 13 , further comprising a promoter that controls expression of the nucleic acid.

16. The host cell of claim 14 , further comprising a promoter that controls expression of the nucleic acid of (a) or the nucleic acid of (b).

17. The host cell of claim 14 , comprising the nucleic acid of (a) and the nucleic acid of (b).

18. The host cell of claim 17 , further comprising a promoter that controls expression of the nucleic acid of (a) and the nucleic acid of (b).

19. The host cell of claim 17 , further comprising a first promoter that controls expression of the nucleic acid of (a) and a second promoter that controls expression of the nucleic acid of (b).

20. The host cell of claim 13 , which is a mammalian host cell selected from the group consisting of 293 cell, 3T3 cell, A549 cell, BHK cell, BT2O T47D cell, BT483 cell, CHO cell, COS cell, CRL7O3O cell, HeLa cell, Hep G2 cell, Hs578T cell, HsS78Bst cell, HTB2 cell, MDCK cell, NS0 cell, SP2 cell, VERY cell, and W138 cell.

21. The host cell of claim 20 , which is a CHO cell.

22. The host cell of claim 13 , which expresses an antibody comprising a heavy chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 113, 121, 137, 157, or 161 and a light chain variable region comprising an amino acid sequence set forth as any one of SEQ ID NOs: 115, 123, 139, 159, or 163.

Assignees

• Stemcentrx Inc
• Abbvie Stemcentrx Llc

Inventors

• Hampl Johannes
• Dylla Scott J
• Foord Orit
• Stull Robert A

Classifications

• A61P35/02

  specific for leukemia · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61P35/04

  specific for metastasis · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• G01N33/575

  for cancer · CPC title