Labeling composition for intraocular tissue, labeling method of intraocular tissue, and screening method

Patent metadata

Field	Value
Publication number	US-9764046-B2
Application number	US-201514601480-A
Country	US
Kind code	B2
Filing date	Jan 21, 2015
Priority date	Dec 25, 2008
Publication date	Sep 19, 2017
Grant date	Sep 19, 2017

Abstract

The invention provides a labeling composition for an intraocular tissue of a living individual, which specifically labels the intraocular tissue without need of an invasive operation such as exposure of an ocular tissue or injection of a staining agent into the ocular tissue or a nerve tissue linking to the ocular tissue, a method of noninvasively labeling an intraocular tissue of a living individual, and a screening method using the labeling composition for the intraocular tissues. The composition contains a compound capable of labeling at least a photoreceptor cell layer of a retina, wherein the compound is a staining compound having a particular structure as a partial structure thereof.

First claim

The invention claimed is:

1. An imaging method for an intraocular tissue comprising:
bringing a labeling composition for the intraocular tissue or a staining agent for the intraocular tissue, which comprises the labeling composition for the intraocular tissue, into contact with the ocular tissue;
irradiating the intraocular tissue with excitation light to observe fluorescence; and
acquiring a fluorescent image,
wherein the labeling composition comprises a fluorescent compound capable of labeling at least a photoreceptor cell layer of a retina, and
wherein the fluorescent compound is selected from the group consisting of compounds (1)-(9), (11)-(13), (32), (33), (87), (90)-(95), and (109)-(112):

2. An evaluating method for a condition of a retina comprising:
imaging an intraocular tissue by the imaging method according to claim 1 ; and
evaluating the condition of the retina using the obtained fluorescent image.

Assignees

• Canon Kk

Inventors

• Watanabe Kohei
• Shintou Taichi
• Nomoto Tsuyoshi
• Miyazaki Takeshi
• Tanaka Toshio
• Nishimura Yuhei
• Shimada Yasuhito
• Nishimura Norihiro

Classifications

• C07D277/36

  Sulfur atoms · CPC title

• C07D277/64

  with only hydrocarbon or substituted hydrocarbon radicals attached in position 2 · CPC title

• C07D491/12

  in which the condensed system contains three hetero rings · CPC title

• G01N33/5088

  of vertebrates · CPC title

• C07D209/96

  Spiro-condensed ring systems · CPC title