Chimeric gene constructs for generation of fluorescent transgenic ornamental fish

Patent metadata

Field	Value
Publication number	US-9763430-B2
Application number	US-201313738704-A
Country	US
Kind code	B2
Filing date	Jan 10, 2013
Priority date	Feb 18, 1999
Publication date	Sep 19, 2017
Grant date	Sep 19, 2017

Abstract

Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

First claim

The invention claimed is:

1. A transgenic fish comprising a chimeric gene comprising a zebrafish acidic ribosomal protein gene promoter operably linked to an exogenous gene, wherein said promoter comprises a nucleic acid sequence that is identical to the sequence of SEQ ID NO: 9, wherein the transgenic fish contains said promoter in germ cells and/or in somatic cells and is capable of breeding to produce viable and fertile transgenic progeny that express the exogenous gene.

2. The transgenic fish of claim 1 , wherein the exogenous gene under the control of said promoter is a fluorescent protein gene.

3. The transgenic fish of claim 2 , wherein said fish is an ornamental fish for the ornamental fish market.

4. The transgenic fish of claim 2 , wherein said fish and progeny of said fish emits green fluorescence when the whole fish is exposed to a blue or ultraviolet light.

5. The transgenic fish of claim 1 , further defined as a transgenic zebrafish, medaka, goldfish, carp, koi, loach, tilapia, glassfish, catfish, angel fish, discus, eel, tetra, goby, gourami, guppy, Xiphophorus, hatchet fish, Molly fish, or pangasius.

6. The transgenic fish of claim 5 , further defined as a transgenic zebrafish, medaka, goldfish or carp.

7. The transgenic fish of claim 6 , further defined as a transgenic zebrafish.

Assignees

• Gong Zhiyuan
• He Jiangyan
• Ju Bensheng
• Lam Toong Jin
• Xu Yanfei
• Yan Tie
• Nat Univ Singapore

Inventors

• Gong Zhiyuan
• He Jiangyan
• Ju Bensheng
• Lam Toong Jin
• Xu Yanfei
• Yan Tie

Classifications

• C12N15/85

  for animal cells · CPC title

• A01K2267/02

  Animal zootechnically ameliorated · CPC title

• C07K14/43595

  from coelenteratae, e.g. medusae · CPC title

• C12N2840/20

  translation of more than one cistron · CPC title

• C12N2830/80

  from vertebrates · CPC title