Artificial kidney precursor and process for production thereof

Patent metadata

Field	Value
Publication number	US-9758766-B2
Application number	US-201414565104-A
Country	US
Kind code	B2
Filing date	Dec 9, 2014
Priority date	Jul 2, 2008
Publication date	Sep 12, 2017
Grant date	Sep 12, 2017

Abstract

The present invention provides an artificial kidney precursor containing a non-human mammalian metanephros separated out from a living body, wherein the metanephros has been subjected to freezing and thawing treatments outside a living body, and contains mammalian mesenchymal stem cells transferred outside a living body, and a method of production thereof.

First claim

What is claimed is:

1. A method of producing an artificial kidney precursor that is to be transplanted to a human recipient, the precursor comprising:
a non-human mammalian metanephros comprising blast cells of a kidney; and
human mesenchymal stem cells isolated from the human recipient, which method comprises the steps of:
(I) freezing the non-human mammalian metanephros comprising blast cells of a kidney, said metanephros being separated out from a non-human mammalian embryo outside the living body;
(II) thawing the frozen mammalian metanephros; and
(III) transferring said human mesenchymal stem cells into the metanephros comprising blast cells of a kidney, outside the living body of the non-human mammalian embryo after the thawing (II), thereby obtaining the artificial kidney precursor.

2. The method according to claim 1 , wherein the metanephros used in (I) is a metanephros separated out from a non-human mammalian embryo in a stage where the expression of WIC is still immature.

3. The method according to claim 1 , wherein the artificial kidney precursor is capable of acquiring a potential for erythropoietin production when transplanted into an omentum of the human recipient.

4. The method according to claim 1 , further comprising transplanting the obtained artificial kidney precursor into the human recipient.

Assignees

• Otsuka Pharma Factory Inc
• Univ Jichi Medical
• The Jikei Univ
• Tokyo Women'S Medical Univ

Inventors

• Kobayashi Eiji
• Yokoo Takashi
• Kai Koutaro

Classifications

• C12N2502/02

  embryonic cells · CPC title

• A61K35/12

  Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00) · CPC title

• C12N5/0686

  Kidney cells · CPC title

• C12N2506/1346

  from mesenchymal stem cells · CPC title

• A61K35/22

  Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland · CPC title