Protein chromatography matrices with hydrophilic copolymer coatings

Patent metadata

Field	Value
Publication number	US-9758542-B2
Application number	US-201214355595-A
Country	US
Kind code	B2
Filing date	Nov 1, 2012
Priority date	Nov 1, 2011
Publication date	Sep 12, 2017
Grant date	Sep 12, 2017

Abstract

A coating of a random copolymer of acrylamide and a second monomer, e.g. glycidoxylmethacrylate, for a silica surface is described. The coating is applied to chromatographic support structures having silica based surfaces. The coating is functionalized to produce protein chromatography matrices that are particularly useful for extracting trace amounts of biomarker molecules from biological samples.

First claim

We claim:

1. An affinity chromatography matrix comprising
a. a solid support, and
b. a surface coating on the solid support, the surface coating comprising a copolymer, the copolymer comprising acrylamide and a second monomer comprising glycidoxylmethacrylate, methacrylate, or 2-(dimethylamino)ethyl methacrylate,
wherein the molar ratio of glycidoxylmethacrylate to acrylamide in the copolymer is 20:1,
wherein the molar ratio of methacrylate to acrylamide in the copolymer is 2:1, and
wherein the molar ratio of 2-(dimethylamino)ethyl methacrylateto acrylamide in the copolymer is 2:1.

2. The matrix of claim 1 , wherein the solid support comprises silicon dioxide.

3. The matrix of claim 1 , wherein the solid support comprises a silicon dioxide particle or a silicon dioxide porous monolith.

4. The matrix of claim 1 , wherein the solid support is a nonporous silicon dioxide particle.

5. The matrix of claim 1 , wherein the solid support is a silicon dioxide particle having a diameter less than or equal to 2 μm.

6. The matrix of claim 1 , the surface coating further comprising a binding ligand.

7. The matrix of claim 6 , wherein the binding ligand is selected from the group consisting of streptavidin, protein A, protein G, an antibody, hexahistidine and a metal affinity agent.

8. A method for purifying or extracting a target molecule from a mixture comprising:
a. contacting the mixture with a matrix comprising a solid support, a surface coating on the solid support, the surface coating comprising a copolymer and a binding ligand, the copolymer comprising acrylamide and a second monomer comprising glycidoxyimethacrylate, methacrylate, or 2-(dimethylarnino)ethyl methacrylate, wherein the binding ligand binds to the target molecule;
wherein the molar ratio of glycidoxlmethacrylate to acrylamide in the copolymer is 20:1, wherein the molar ratio of methacrylate to acrylamide in the copolymer is 2:1, and
wherein the molar ratio of 2-(dimethylamino)ethyl methacrylateto acrylamide in the copolymer is 2:1; and

b. recovering the target molecule by contacting the matrix with an eluent which releases the target molecule from the ligand.

9. The method of claim 8 , further comprising between steps a and b, contacting the matrix with a wash solution capable of removing unbound substances from the matrix.

10. The method of claim 8 , wherein the mixture is blood serum, cell lysates, extracts of blood serum, extracts of cell lysates or protein pharmaceuticals.

11. The method of claim 8 , wherein the target molecule comprises proteins, peptides, nucleic acids, glycoproteins and sugars.

12. The method of claim 8 , wherein the method is able to purify or extract a target molecule from a mixture present in less than 500 pM.

13. The method of claim 8 , wherein the method is able to purify or extract a target molecule form a mixture present in less than 50 fmol.

14. The matrix of claim 1 , wherein the solid support is a silicon dioxide particle having a diameter between 50 nm and 500 nm.

15. The matrix of claim 1 , wherein the solid support is a silicon dioxide particle having a diameter between 50 nm and 200 nm.

16. The matrix of claim 1 , wherein the solid support is a silicon dioxide particle having a diameter between 300 nm and 500 nm.

Assignees

• Purdue Research Foundation

Inventors

• Wirth Mary J
• Hua Yimin
• Zhang Zhaorui

Classifications

• B01D15/3809

  of the antigen-antibody type, e.g. protein A, G or L chromatography · CPC title

• B01J20/3204

  Inorganic carriers, supports or substrates · CPC title

• B01J20/327

  Polymers obtained by reactions involving only carbon to carbon unsaturated bonds · CPC title

• B01J20/283

  based on silica · CPC title

• B01J20/28016

  Particle form · CPC title