Silicone surfactants for emulsion assays

Patent metadata

Field	Value
Publication number	US-9758535-B2
Application number	US-201414216006-A
Country	US
Kind code	B2
Filing date	Mar 17, 2014
Priority date	Mar 15, 2013
Publication date	Sep 12, 2017
Grant date	Sep 12, 2017

Abstract

System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

First claim

We claim:

1. A composition comprising at least one silicone surfactant having the formula:

the formula

or the formula

wherein
each g is independently 5-200;
each h is independently 1-5;
each i is independently 1-5;
each j is independently 15-50; and
each k is independently 2-100.

2. The composition of claim 1 , wherein the composition is an emulsion of aqueous droplets in a silicone oil phase.

3. The composition of claim 2 , wherein the silicone oil phase includes one or more of octamethyl trisiloxane and a polydimethylsiloxane polymer.

4. The composition of claim 2 , wherein the at least one silicone surfactant is present in the composition at a concentration of 0.1% to 10% in the silicone oil phase.

5. The composition of claim 2 , wherein the aqueous droplets include a photoluminescent reporter.

6. The composition of claim 2 , wherein the aqueous droplets include an analyte that is a nucleic acid.

7. The composition of claim 2 , wherein the silicone oil phase includes octamethyl trisiloxane, cyclopentasiloxane or a trimethylsiloxysilicate at a concentration of about 0.1% to 5%.

8. The composition of claim 7 , wherein the at least one silicone surfactant is present at a concentration of about 0.2% to 5% by weight in the silicone oil phase.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Chia Erin R
• Hiddessen Amy L
• Hindson Benjamin J
• Lowe Adam
• Han Chunxiao

Classifications

• C08G77/46 Primary

  containing polyether sequences · CPC title

• G01N33/5436

  with ligand physically entrapped within the solid phase (liposomes G01N33/5432; immunological test elements G01N33/54386) · CPC title

• C07F7/184 Primary

  Chemistry & Metallurgy · mapped topic

• C08L83/14

  in which at least two but not all the silicon atoms are connected by linkages other than oxygen atoms (C08L83/10 takes precedence) · CPC title

• C08G77/50

  by carbon linkages · CPC title