Microbial strains, compositions, and methods for increasing available phosphate for plants

Patent metadata

Field	Value
Publication number	US-9758438-B2
Application number	US-201615154278-A
Country	US
Kind code	B2
Filing date	May 13, 2016
Priority date	Nov 16, 2012
Publication date	Sep 12, 2017
Grant date	Sep 12, 2017

Abstract

The present invention provides novel phosphate-solubilizing fungal strains, compositions comprising novel phosphate-solubilizing fungal strains, and methods of using novel phosphate-solubilizing fungal strains to increase the availability of phosphate for plant uptake in soil. In some embodiments, one or more of the novel phosphate-solubilizing fungal strains is coated onto a seed.

First claim

The invention claimed is:

1. A composition comprising:
a soil-compatible carrier; and
one or more fungal strains, said one or more fungal strains comprising the Penicillium bilaiae strain having the deposit accession number NRRL B-50777, the Penicillium bilaiae strain having the deposit accession number NRRL B-50778, the Penicillium bilaiae strain having the deposit accession number NRRL B-50779, the Penicillium bilaiae strain having the deposit accession number NRRL B-50780, the Penicillium bilaiae strain having the deposit accession number NRRL B-50781, the Penicillium bilaiae strain having the deposit accession number NRRL B-50782, the Penicillium bilaiae strain having the deposit accession number NRRL B-50783, the Penicillium bilaiae strain having the deposit accession number NRRL B-50784, the Penicillium bilaiae strain having the deposit accession number NRRL B-50785, the Penicillium bilaiae strain having the deposit accession number NRRL B-50786, the Penicillium bilaiae strain having the deposit accession number NRRL B-50787 and/or the Penicillium bilaiae strain having the deposit accession number NRRL B-50788.

2. The composition of claim 1 , wherein said composition is a powder.

3. The composition of claim 1 , wherein said soil-compatible carrier comprises peat.

4. The composition of claim 1 , wherein said one or more fungal strains comprises fungal spores produced by the Penicillium bilaiae strain having the deposit accession number NRRL B-50777, the Penicillium bilaiae strain having the deposit accession number NRRL B-50778, the Penicillium bilaiae strain having the deposit accession number NRRL B-50779, the Penicillium bilaiae strain having the deposit accession number NRRL B-50780, the Penicillium bilaiae strain having the deposit accession number NRRL B-50781, the Penicillium bilaiae strain having the deposit accession number NRRL B-50782, the Penicillium bilaiae strain having the deposit accession number NRRL B-50783, the Penicillium bilaiae strain having the deposit accession number NRRL B-50784, the Penicillium bilaiae strain having the deposit accession number NRRL B-50785, the Penicillium bilaiae strain having the deposit accession number NRRL B-50786, the Penicillium bilaiae strain having the deposit accession number NRRL B-50787 and/or the Penicillium bilaiae strain having the deposit accession number NRRL B-50788.

5. The composition of claim 1 , wherein said composition further comprises one or more phosphorous sources.

6. The composition of claim 1 , wherein said composition further comprises rock phosphate, monoammonium phosphate, diammonium phosphate, monocalcium phosphate, super phosphate, triple super phosphate, ammonium polyphosphate, bone meal, meat meal, animal manure, compost, sewage sludge and/or guano.

7. The composition of claim 1 , wherein said composition further comprises one or more lipo-chitooligosaccharides.

8. The composition of claim 1 , wherein said composition further comprises one or more chitooligosaccharides.

9. The composition of claim 1 , wherein said composition further comprises one or more chitins.

10. The composition of claim 1 , wherein said composition further comprises one or more chitosans.

11. The composition of claim 1 , wherein said composition further comprises one or more flavonoids.

12. The composition of claim 1 , wherein said composition further comprises luteolin, apigenin, tangeritin, quercetin, kaempferol, myricetin, fisetin, isorhamnetin, pachypodol, rhamnazin, hesperetin, naringenin, eriodictyol, homoeriodictyol, taxifolin, dihydroquercetin, dihydrokaempferol, genistein, daidzein, glycitein, catechin, gallocatechin, catechin 3-gallate, gallocatechin 3-gallate, epicatechin, epigallocatechin, epicatechin 3-gallate, epigallocatechin 3-gallate, cyanidin, delphinidin, malvidin, pelargonidin, peonidin or petunidin.

13. The composition of claim 1 , wherein said composition further comprises one or more jasmonic acids, linoleic acids, linolenic acids and/or karrakins.

14. The composition of claim 1 , wherein said composition further comprises one or more diazotrophic microorganisms.

15. The composition of claim 1 , wherein said composition further comprises one or more mycorrhizae.

16. The composition of claim 1 , wherein said composition further comprises one or more herbicides, fungicides and/or insecticides.

17. The composition of claim 1 , wherein said composition further comprises azoxystrobin, clothianidin, fludioxonil, fomesafen, glufosinate, glyphosate, imidacloprid, lactofen, mefenoxam, metalaxyl and/or thiamethoxam.

18. A seed treated with the composition of claim 1 .

19. The seed of claim 18 , wherein said seed is coated with 1×10 1 to 1×10 8 colony-forming units of said one or more fungal strains.

20. A container comprising a plurality of seeds treated with the composition of claim 1 .

Assignees

• Novozymes Bioag As

Inventors

• Greenshields David
• Steckler Shelagh
• Priest Kari
• Caldwell Caressa
• Frodyma Michael

Classifications

• C05G3/60

  Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants · CPC title

• A01N63/30 Primary

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• C12N1/14 Primary

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title

• A01H3/00

  Processes for modifying phenotypes {, e.g. symbiosis with bacteria} (A01H4/00 takes precedence) · CPC title

• C05G3/00

  Mixtures of one or more fertilisers with additives not having a specially fertilising activity · CPC title