Sample analyzing method using sample analysis cartridge, sample analysis cartridge, and sample analyzer

Patent metadata

Field	Value
Publication number	US-9752989-B2
Application number	US-201615082337-A
Country	US
Kind code	B2
Filing date	Mar 28, 2016
Priority date	Apr 30, 2015
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

A sample analyzing method for suppressing an adverse effect on detection accuracy of a detection target substance is disclosed. In the sample analyzing method, a magnetic particle as a support of a detection target substance is transported by magnetic force to a second liquid container through a passage between a first liquid container storing a first liquid containing the magnetic particle and the second liquid container storing a second liquid containing a labeled substance to forma complex with the detection target substance and the magnetic particle. The complex formed in the second liquid container and containing the detection target substance, magnetic particle, and labeled substance is transported to a third liquid in a flow path, and the magnetic particle is transported to a detection tank for detecting the detection target substance, while being agitated in a mixed liquid of the complex and third liquid within the flow path.

First claim

The invention claimed is:

1. A sample analyzing method using a sample analysis cartridge inserted into a sample analyzer that detects a detection target substance contained in a sample, comprising:
transporting a magnetic particle supporting the detection target substance by magnetic force from a first liquid container to a second liquid container through a passage disposed between the first and second liquid containers, the first liquid container storing a first liquid containing the magnetic particle to be a support of the detection target substance, the second liquid container storing a second liquid containing a labeled substance which is to form a complex together with the detection target substance and the magnetic particle;
transporting the complex, which is formed in the second liquid container, and contains the detection target substance, the magnetic particle, and the labeled substance, to a third liquid in a flow path; and
transporting the magnetic particle to a detection tank to detect the detection target substance, while agitating the magnetic particle in a mixed liquid of the complex and the third liquid within the flow path.

2. The sample analyzing method using a sample analysis cartridge according to claim 1 , wherein
the magnetic particle is transported while being agitated in the mixed liquid within the flow path by an air pressure.

3. The sample analyzing method using a sample analysis cartridge according to claim 1 , wherein
the magnetic particle is agitated in the mixed liquid in such a way that the mixed liquid is moved back and forth within the flow path by an air pressure.

4. The sample analyzing method using a sample analysis cartridge according claim 1 , wherein
the flow path meanders, and
the magnetic particle is agitated in the mixed liquid in such a way that the mixed liquid is moved in the meandering flow path.

5. The sample analyzing method using a sample analysis cartridge according to claim 4 , wherein
the mixed liquid is moved within the flow path also in a thickness direction of the sample analysis cartridge.

6. The sample analyzing method using a sample analysis cartridge according to claim 1 , wherein
a fourth liquid containing a substrate that facilitates light emission by reacting with the complex is transported to the detection tank by an air pressure.

7. The sample analyzing method using a sample analysis cartridge according to claim 6 , wherein
the fourth liquid is transported to a position in the detection tank near a position to which the mixed liquid is transported from the flow path.

8. A sample analysis cartridge to be inserted into a sample analyzer that detects a detection target substance contained in a sample, comprising:
a first liquid container that stores a first liquid containing a magnetic particle to be a support of the detection target substance;
a second liquid container that stores a second liquid containing a labeled substance which is to form a complex together with the detection target substance and the magnetic particle;
a passage that is disposed between the first liquid container and the second liquid container, and that transports the magnetic particle supporting the detection target substance to the second liquid container by magnetic force; and

a first flow path that transports the complex, which is formed in the second liquid container and contains the detection target substance, the magnetic particle, and the labeled substance, to a third liquid, wherein the magnetic particle is transported to a detection tank to detect the detection target substance, and the magnetic particle is configured to be agitated in a mixed liquid of the complex and the third liquid in the first flow path while being transported to the detection tank.

9. The sample analysis cartridge according to claim 8 , wherein
the magnetic particle is transported while being agitated in the mixed liquid in the first flow path by an air pressure.

10. The sample analysis cartridge according to claim 8 , further comprising:
an air chamber that transports the magnetic particle to the detection tank while agitating the magnetic particle in the mixed liquid in the first flow path by an air pressure.

11. The sample analysis cartridge according to claim 10 , wherein
the air chamber is alternately deformed between an initial state and a contracted state, thereby moving the mixed liquid back and forth in the first flow path and agitating the magnetic particle in the mixed liquid.

12. The sample analysis cartridge according to claim 8 , wherein
the first flow path is formed in a meandering shape.

13. The sample analysis cartridge according to claim 8 , wherein
a volume of the first flow path is larger than a volume of the mixed liquid.

14. The sample analysis cartridge according to claim 12 , wherein
the first flow path is formed to also extend in a thickness direction of the sample analysis cartridge.

15. The sample analysis cartridge according to claim 14 , wherein
the first flow path includes a first portion and a second portion disposed at positions lower than the detection tank in the thickness direction,
the first portion is formed to extend in a direction perpendicular to the thickness direction, and
the second portion is formed to extend in the thickness direction and to include a first end connected to the first portion and a second end connected to the detection tank.

16. The sample analysis cartridge according to claim 8 , wherein
the first flow path is disposed on an extension of the passage in a transportation direction of the magnetic particle in the passage.

17. The sample analysis cartridge according to claim 8 , further comprising:
an air chamber that transports the magnetic particle to the detection tank while agitating the magnetic particle in the mixed liquid in the first flow path by an air pressure, the air chamber being a first air chamber that transports the mixed liquid to the detection tank from the first flow path; and
a second air chamber that transports a fourth liquid to the detection tank by an air pressure, the fourth liquid containing a substrate that facilitates light emission by reacting with the complex.

18. The sample analysis cartridge according to claim 17 , further comprising a second flow path that is connected to the detection tank and that transports the fourth liquid to the detection tank,
wherein the second flow path is connected to a portion of the detection tank near the first flow path connected to the detection tank.

19. A sample analyzer that performs a sample analysis using a sample analysis cartridge, comprising:
a first liquid container that stores a first liquid containing a magnetic particle to be a support of a detection target substance;
a second liquid container that stores a second liquid containing a labeled substance which is to form a complex together with the detection target substance and the magnetic particle;
a passage that is disposed between the first liquid container and the second liquid container, and that transports the magnetic particle supporting the detection target substance to the second liquid container by magnetic force; and

a flow path that transports the complex formed in the second liquid container, and contains the detection target substance, the magnetic particle, and the labeled substance, to a third liquid, wherein the sample analysis is performed using the sample analysis cartridge in which the magnetic particle is transported from inside of the flow path to a detect

Assignees

• Sysmex Corp

Inventors

• Horii Kazuyoshi
• Nose Tomoyuki
• Kosako Tatsuya

Classifications

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• B03C1/02

  acting directly on the substance being separated · CPC title

• Y10T436/111666

  Utilizing a centrifuge or compartmented rotor · CPC title

• B01L2400/0406

  capillary forces · CPC title