Microfluidic devices

Patent metadata

Field	Value
Publication number	US-9752185-B2
Application number	US-201514824333-A
Country	US
Kind code	B2
Filing date	Aug 12, 2015
Priority date	Sep 15, 2004
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense. Also disclosed in the present disclosure is a flow through, traveling-wave, bead-beating device which comprises a rotating pole piece, a flow through tube, and a magnetic piece. Rotation of the rotating pole piece may create a magnetic wave down the flow through tube, thereby producing sufficient acceleration of beads through the tube to disrupt or lyse target analytes flowing through the tube.

First claim

What is claimed is:

1. A traveling magnetic wave flow through device, comprising:
a rotating pole piece;
a flow through tube;
a magnetic fixed piece; and
at least one bead positioned in the lumen of said flow through tube, wherein said flow through tube is in fluidic communication with a microfluidic device, and wherein said rotating pole piece is configured to produce a travelling magnetic wave down said flow through tube upon rotation of said rotating pole piece.

2. The device of claim 1 , wherein the rotation of said rotating pole piece is at least about 100 Hz.

3. The device of claim 1 , wherein said flow through tube is adjacent to said rotating pole piece.

4. The device of claim 3 , wherein said flow through tube is axially parallel to said rotating pole piece.

5. The device of claim 3 , wherein said flow through tube is fluidically separated from said rotating pole piece.

6. The device of claim 1 , further comprising wherein said at least one bead comprises a plurality of beads positioned in the lumen of said flow through tube.

7. The device of claim 6 , wherein said plurality of beads comprises magnetic beads.

8. The device of claim 7 , wherein said rotation of said rotating pole piece produces acceleration of said magnetic beads to lyse target analytes flowing through said flow through tube.

Assignees

• Integenx Inc

Inventors

• Boronkay Allen
• Jovanovich Stevan Bogdan
• Blaga Iuliu Ioan

Classifications

• C12N15/1013

  by using magnetic beads · CPC title

• C12N15/1003

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title

• B01L2200/028

  Modular arrangements · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L2300/0887

  Laminated structure · CPC title