Non-caloric sweetener
US-2016208303-A1 · Jul 21, 2016 · US
US9752174B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9752174-B2 |
| Application number | US-201414287837-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 27, 2014 |
| Priority date | May 28, 2013 |
| Publication date | Sep 5, 2017 |
| Grant date | Sep 5, 2017 |
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Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2 and reb M2 are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.
Opening claim text (preview).
We claim: 1. A method for preparing reb D2 comprising: a. contacting a starting composition comprising reb A with UDP-glucosyltransferase, to produce a composition comprising reb D2; and b. isolating the composition comprising reb D2. 2. The method of claim 1 , further comprising purifying the composition comprising reb D2 to provide reb D2 having a purity greater than about 95% by weight on an anhydrous basis. 3. A method for preparing reb M2 comprising: a. contacting a starting composition comprising reb D2 with UDP-glucosyltransferase to produce a composition comprising reb M2; and b. isolating the composition comprising reb M2. 4. The method of claim 3 , further comprising purifying the composition comprising reb M2 to provide reb M2 having a purity greater than about 95% by weight on an anhydrous basis.
Polyterpene radicals · CPC title
by natural substances obtained from animals or plants · CPC title
containing sugars or derivatives · CPC title
having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin · CPC title
Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title
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