Compositions and methods for inhibiting terminal transferase activity

US9752173B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9752173-B2
Application numberUS-201514754262-A
CountryUS
Kind codeB2
Filing dateJun 29, 2015
Priority dateApr 8, 2010
Publication dateSep 5, 2017
Grant dateSep 5, 2017

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  1. Title

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  2. Abstract

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  5. First independent claim

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Abstract

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The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification techniques having reduced 3′ non-templated nucleotide addition.

First claim

Opening claim text (preview).

We claim: 1. A kit comprising a PCR reaction solution, wherein said PCR reaction solution comprises boric acid, deoxynucleotide triphosphates, divalent cations, KCl and Tris-HCl or (NH) 4 SO 4 and Tris-HCl, and a polymerase having terminal transferase activity. 2. The kit of claim 1 , wherein said divalent cations are selected from group consisting of Mg 2+ and Mn 2+ divalent cations. 3. The kit of claim 1 , wherein said polymerase having terminal transferase activity is Taq polymerase or a fragment thereof.

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What does patent US9752173B2 cover?
The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification …
Who is the assignee on this patent?
Ibis Biosciences Inc, Hofstadler Nina M
What technology area does this patent fall under?
Primary CPC classification C07K14/4703. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Sep 05 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).