Peptides and methods for reducing skin pigmentation
US-2024190917-A1 · Jun 13, 2024 · US
US9752173B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9752173-B2 |
| Application number | US-201514754262-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 29, 2015 |
| Priority date | Apr 8, 2010 |
| Publication date | Sep 5, 2017 |
| Grant date | Sep 5, 2017 |
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The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification techniques having reduced 3′ non-templated nucleotide addition.
Opening claim text (preview).
We claim: 1. A kit comprising a PCR reaction solution, wherein said PCR reaction solution comprises boric acid, deoxynucleotide triphosphates, divalent cations, KCl and Tris-HCl or (NH) 4 SO 4 and Tris-HCl, and a polymerase having terminal transferase activity. 2. The kit of claim 1 , wherein said divalent cations are selected from group consisting of Mg 2+ and Mn 2+ divalent cations. 3. The kit of claim 1 , wherein said polymerase having terminal transferase activity is Taq polymerase or a fragment thereof.
Terminal transferase · CPC title
being a mass spectrometer · CPC title
Specific component of sample, medium or buffer · CPC title
IL-4 · CPC title
Inhibitors; Suppressors · CPC title
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