Compositions comprising a polypeptide having cellulolytic enhancing activity and a quinone compound and uses thereof

Patent metadata

Field	Value
Publication number	US-9752168-B2
Application number	US-201615056404-A
Country	US
Kind code	B2
Filing date	Feb 29, 2016
Priority date	Aug 12, 2010
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a quinone compound. The present invention also relates to methods of using the compositions.

First claim

What is claimed is:

1. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity and a quinone compound, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the quinone compound enhances hydrolysis of the cellulosic material by the enzyme composition compared to the GH61 polypeptide alone or the quinone compound alone according to the following formula with a ratio greater than 1:

GH
⁢

⁢
61
⁢

⁢
effect

=

%
⁢

⁢

conversion

(

+
GH

⁢

⁢
61

+

quinone
⁢

⁢
compound

)

%
⁢

⁢

conversion

(

no
⁢

⁢
GH
⁢

⁢
61

+

⁢

quinone
⁢

⁢
compound

)

;

(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.

2. The method of claim 1 , wherein the quinone compound is a compound of formula (I) or (II):

wherein R 1 , R 2 , R 3 , and R 4 are independently hydrogen, halogen, —OH, —OR 8 , —NO 2 , —N(R 9 )(R 10 ), —CN, —C(O)R 5 , —C(O)OR 8 , —C(O)NHR 7 , —OC(O)R 11 , —NHC(O)R 12 , —OC(O)OR 13 , —NHC(O)OR 14 , —OC(O)NHR 15 , —NHC(O)NHR 16 , —SO 2 R 17 , —SO 2 N(R 18 )(R 19 ), —SR 20 , —NC(R 21 )(R 22 ), or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 , R 18 , R 19 , R 20 , R 21 , and R 22 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and
R 17 is an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and
wherein each pair of R 1 and R 2 , R 2 and R 3 , and R 3 and R 4 may combine to form an optionally substituted fused ring;
or a salt or solvate thereof.

3. The method of claim 2 , wherein the quinone compound is selected from the group consisting of: (I-1): 1,4-benzoquinone; (I-2): 1,4-naphthoquinone; (I-3): 2-hydroxy-1,4-naphthoquinone; (I-4): 2,3-dimethoxy-5-methyl-1,4-benzoquinone or coenzyme Q 0 ; (I-5): 2,3,5,6-tetramethyl-1,4-benzoquinone or duroquinone; (I-6): 1,4-dihydroxyanthraquinone; (II-1): 3-hydroxy-1-methyl-5,6-indolinedione or adrenochrome; (II-2): 4-tert-butyl-5-methoxy-1,2-benzoquinone; and (II-3): pyrroloquinoline quinone; or a salt or solvate thereof.

4. The method of claim 1 , wherein the cellulosic material is pretreated.

5. The method of claim 1 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

6. The method of claim 5 , wherein the cellulase is one or more enzymes selected from the group consisting of an endoglucanase, a cellobiohydrolase, and a beta-glucosidase.

7. The method of claim 5 , wherein the hemicellulase is one or more enzymes selected from the group consisting of a xylanase, an acetyxylan esterase, a feruloyl esterase, an arabinofuranosidase, a xylosidase, and a glucuronidase.

8. The method of claim 1 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.

9. The method of claim 1 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is about 10 −6 to about 10.

10. The method of claim 1 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is about 10 −6 to about 7.5.

11. The method of claim 1 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is about 10 −6 to about 5.

12. The method of claim 1 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is about 10 −6 to about 2.5.

13. The method of claim 1 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is about 10 −6 to about 1.

14. The method of claim 1 , wherein an effective amount of the quinone compound to cellulosic material as a molar ratio to glucosyl units of cellulose is abou

Assignees

• Novozymes Inc

Inventors

• Quinlan Jason
• Xu Feng
• Sweeney Matthew

Classifications

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12P5/026

  Unsaturated compounds, i.e. alkenes, alkynes or allenes · CPC title

• C12N9/2437

  Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title

• C12P7/28

  Acetone-containing products · CPC title