Antibodies to risperidone haptens and use thereof

Patent metadata

Field	Value
Publication number	US-9751953-B2
Application number	US-201313971475-A
Country	US
Kind code	B2
Filing date	Aug 20, 2013
Priority date	Aug 21, 2012
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

Disclosed is an antibody which binds to risperidone, which can be used to detect risperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of risperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.

First claim

What is claimed is:

1. A method of producing an antibody which binds to risperidone, the method comprising:
(i) selecting a host for antibody production; and
(ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier, wherein the host produces the antibody which binds to risperidone,
Formula I:

wherein:
R 1 is H, or OH;
R 2 is

or O(CH 2 ) r NHC(O)(CH 2 ) m CO 2 H;
wherein:
r is 1, 2, 3, 4, or 5;
m is 1, 2, 3, 4, or 5; and
n is 1, 2, 3, 4, or 5.

2. The method of claim 1 , wherein the antibody is an antibody binding fragment which is selected from the group of fragments consisting of Fv, F(ab′), F(ab′)2, scFv, minibody and diabody fragments.

3. The method of claim 1 , wherein the antibody is a monoclonal antibody.

4. The method of claim 1 , wherein the immunogenic carrier is a protein.

5. The method of claim 4 , wherein the protein is keyhole limpet hemocyanin, bovine thyroglobulin, or ovalbumin.

6. A method of producing a hybridoma cell line, which produces a monoclonal antibody which binds to risperidone, the method comprising:
(i) selecting a host for antibody production;
(ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier;
(iii) fusing a cell line from said inoculated host, wherein the cell line produces antibodies that bind to risperidone, with a continuously dividing cell to create a fused cell, which produces a monoclonal antibody which binds to risperidone; and
(iv) cloning the fused cell so as to obtain a hybridoma cell line,
Formula I:

wherein:
R 1 is H, or OH;
R 2 is

or O(CH 2 ) r NHC(O)(CH 2 ) m CO 2 H;
wherein:
r is 1, 2, 3, 4, or 5;
m is 1, 2, 3, 4, or 5; and
n is 1, 2, 3, 4, or 5.

7. The method of claim 6 , wherein the immunogenic carrier is a protein.

8. The method of claim 7 , wherein the protein is keyhole limpet hemocyanin, bovine thyroglobulin, or ovalbumin.

Assignees

• Janssen Pharmaceutica Nv

Inventors

• Hryhorenko Eric
• Sankaran Banumathi
• Decory Thomas R
• Tubbs Theresa
• Colt Linda
• Vliegen Maarten
• Haspeslagh Pieter Rik

Classifications

• A61P25/18

  Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia · CPC title

• A61P25/24

  Antidepressants · CPC title

• C07K16/44 Primary

  against material not provided for elsewhere {, e.g. haptens, metals, DNA, RNA, amino acids} · CPC title

• G01N33/94

  involving narcotics {or drugs or pharmaceuticals, neurotransmitters or associated receptors} · CPC title

• G01N2800/302

  Schizophrenia · CPC title