Biological culture assembly

Patent metadata

Field	Value
Publication number	US-9751084-B2
Application number	US-58936909-A
Country	US
Kind code	B2
Filing date	Oct 22, 2009
Priority date	Oct 28, 2008
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

The invention relates to a single use cell culture assembly for use in carrying out bioreactions and growing microorganisms, and tissue and cell cultures. The cell culture assembly is designed to receive an insert having a microscope slide upper surface, and includes a well frame having a plurality of partitioned well compartments, adapted to be positioned on the upper surface of the insert, and includes a liquid-impermeable releasable seal positioned on or within the lower edge of the well frame for maintaining a liquid-impermeable barrier between well compartments.

First claim

What is claimed is:

1. A cell culture assembly system comprising:
a) a base member insert having an upper surface and a length; and
b) a cell culture assembly having,
i) a bottom support frame for receiving a first portion of said the base member insert less than said length, such that a second portion of said base member insert extends outwardly from said bottom support frame, said bottom support frame comprising a pair of opposite side walls and a cross member support connecting said opposite side walls, said opposite side walls defining between them a base member insert supporting surface that includes said cross member support, said base member insert being receivable between said pair of opposite side walls and being supportable by said supporting surface;
ii) a well compartment frame having sidewalls, each sidewall having an upper and lower surface, and one or more well compartments, each well compartment having an upper and a lower well compartment opening;
iii) a sealing element positioned on or within the lower surface of the well compartment frame adapted to operatively create a liquid-impermeable releasable seal between the upper surface of the first portion of the base member insert and the lower surface of the well compartment frame when the well frame is positioned on the upper surface of the first portion of the base member insert and the liquid-impermeable seal is compressed onto or otherwise sealingly engaged with the upper surface of the first portion of the base member insert, such that when the well compartment frame and base member insert are physically separated, the sealing element leaves the upper surface of the first portion of said base member insert free of sealing element material,
iv) a fastening mechanism comprising a first element located on the support frame and a second element located on the well compartment frame, the first and second elements cooperatively engaging with one another for fastening the support frame to the well compartment frame when the assembly is in a closed position; and
v) a removable cover adapted to be positioned over the upper well compartment opening, while also providing for air/gas exchange to maintain the pH of the culture medium and liquid reagents contained in each well compartment.

2. The assembly of claim 1 , wherein the sealing element comprises an integral over-molded material selected from the group consisting of elastomers, thermoplastics, synthetic rubber, natural rubber or mixtures thereof.

3. The assembly of claim 1 , wherein the sealing element comprises a material selected from the group consisting of elastomers, thermoplastics, synthetic rubber, natural rubber or mixtures thereof, and the sealing element is adhesively bonded to the lower surface of the well frame.

4. The assembly of claim 1 , wherein the lower surface of the well compartment frame further comprises a groove for receiving the sealing element, and the sealing element comprises a material selected from the group consisting of elastomers, thermoplastics, synthetic rubber, natural rubber or mixtures thereof.

5. The assembly of claim 1 , wherein the cover further comprises (1) a plurality of stacking ribs incorporated onto the top outer surface of the cover, thereby allowing multiple assemblies to be arranged in a stacked configuration one on top of the other, and (2) elongated ridges located on the underside of the cover thereby enclosing each well compartment opening to minimize contamination and evaporation in each well compartment.

6. The assembly of claim 1 , further comprising a hinged attachment element for securing the support frame to the well compartment frame.

7. The assembly of claim 1 , wherein the base member insert is a microscope slide.

8. The assembly of claim 1 , wherein the base member insert is glass or plastic.

9. The assembly of claim 8 , wherein the base member insert is plastic and further comprises:
a) one or more through holes covered with a membrane, wherein each through hole is sealed liquid tight along the hole perimeter, and each through hole is in positional alignment with a well compartment in the well compartment frame.

10. A cell culture assembly system comprising:
a) a base member insert having an upper surface and a length; and
b) a one-piece cell culture assembly having,
i) a bottom support frame for receiving a first portion of the base member insert less than said length, such that a second portion of said base member insert extends outwardly from said bottom support frame, said bottom support frame comprising a pair of opposite side walls and a cross member support connecting said opposite side walls, said opposite side walls defining between them a base member insert supporting surface that includes said cross member support, said base member insert being receivable between said pair of opposite side walls and being supportable by said supporting surface;
ii) a well compartment frame having sidewalls, each sidewall having an upper and lower surface, and one or more well compartments, each well compartment having an upper and a lower well compartment opening;
iii) a sealing element comprising an integral over-molded material selected from the group consisting of elastomers, thermoplastics, synthetic rubber, natural rubber or mixtures thereof, positioned on or within the lower surface of the well compartment frame adapted to operatively create a liquid-impermeable releasable seal between the upper surface of the first portion of the base member insert and the lower surface of the well compartment frame when the well compartment frame is positioned on the upper surface of the first portion of the base member insert and the liquid-impermeable seal is compressed onto or otherwise sealingly engaged with the upper surface of the first portion of the base member insert, such that when the well compartment frame and base member insert are physically separated, the sealing element leaves the upper surface of the first portion of the base member insert free of sealing element material,
iv) a fastening mechanism comprising a first element located on the support frame and a second element located on the well compartment frame, the first and second elements cooperatively engaging with one another for fastening the support frame to the well compartment frame when the assembly is in a closed position; and
v) a hinged attachment element for hingedly securing the support frame to the well compartment frame; and

c) a removable cover having an underside, adapted to be positioned over the upper well compartment openings, and having elongated ridges located on the underside of the cover thereby enclosing each well compartment opening to minimize contamination and evaporation in each well compartment, while also providing for air/gas exchange to maintain the pH of the culture medium and liquid reagents contained in each well compartment.

11. A method for growing biological cultures using the assembly of claim 10 comprising the steps:
a) positioning the base member insert onto the support frame;
b) positioning the well compartment frame having the sealing element located on the lower surface onto the upper surface of the base member insert such that the sealing element creates a liquid-impermeable seal between the lower surface of the well compartment frame and the upper surface of the first portion of the base member insert, and thereby forming one or more well compartments on the upper surface of the first portion of the base member insert;
c) placing a biological culture medium into each individual well compartment onto the upper surface of the first portion of the base member insert;
d) introducing a biological test sample liquid

Assignees

• Greenizen Kurt E
• Clark Phillip
• Doyle John J
• Emd Millipore Corp

Inventors

• Greenizen Kurt E
• Clark Phillip
• Doyle John J

Classifications

• B01L2200/0689

  Sealing · CPC title

• B01L3/5085 Primary

  for multiple samples, e.g. microtitration plates · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title

• B01L2200/028

  Modular arrangements · CPC title

• B01L3/50853

  with covers or lids (closures for test tubes B01L3/50825) · CPC title