Target substance transfer method, crystal production method, composition production method, and target substance transfer device

Patent metadata

Field	Value
Publication number	US-9751068-B2
Application number	US-201213980212-A
Country	US
Kind code	B2
Filing date	Jan 18, 2012
Priority date	Jan 18, 2011
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

The present invention provides a target substance transfer method, a crystal production method, a composition production method, and a target substance transfer device, which allow the concentration of a target substance to be increased easily and effectively. The target substance transfer method is a method for transferring a target substance 103 from a first phase 101 that is a liquid or solid phase containing the target substance 103 to a second phase 102 including: a phase approximation step of bringing the first phase 101 and the second phase 102 into close proximity; and a bubble collapse step of forming bubbles in the vicinity of a boundary between the first phase 101 and the second phase 102 and then causing the bubbles to collapse.

First claim

The invention claimed is:

1. A method for transferring a target substance from a first phase to a second phase, the method comprising:
a phase approximation step of bringing the first phase and the second phase into close proximity; and
a bubble collapse step of forming a bubble in a vicinity of a boundary between the first phase and the second phase and then causing the bubble to collapse,
wherein the first phase is a liquid phase and contains the target substance,
the second phase is a liquid phase or a gel phase, and
in the bubble collapse step, the bubble is formed by focusing a laser beam in the vicinity of the boundary between the first phase and the second phase, and thereby the target substance is transferred from the first phase to the second phase.

2. The method according to claim 1 , wherein the target substance is an organic substance.

3. The method according to claim 2 ,
wherein the organic substance is at least one material selected from the group consisting of proteins, which are native proteins or artificial proteins, peptides, which are native peptides or artificial peptides, nucleic acids, which are native nucleic acids or artificial nucleic acids, lipids, which are native lipids or artificial lipids, carbohydrate chains, which are native carbohydrate chains or artificial carbohydrate chains, high molecular weight organic compounds, low molecular weight organic compounds, and biological substances, which are high molecular weight biological compounds or low molecular weight biological compounds.

4. The method according to claim 1 , wherein
the phase approximation step is a step of bringing the first phase and the second phase into contact with each other to form an interface therebetween.

5. The method according to claim 4 ,
wherein the bubble collapse step is a bubble collision step of forming the bubble in the vicinity of the interface to allow the bubble so as to collide the bubble against the interface.

6. The method according to claim 1 ,
wherein a distance from a focal point of the laser beam to a surface of the second phase on the first phase side is not more than 4 times a maximum radius of the bubble.

7. The method according to claim 1 , wherein
a total irradiation energy of the laser beam is at least 60 nJ.

8. A method for producing a crystal, the method comprising:
a target substance transfer step of transferring a target substance to be crystallized; and
a crystal precipitation step,
wherein the target substance transfer step is the step of transferring the target substance from the first phase containing the target substance to the second phase according to claim 1 , and
the crystal precipitation step is a step of, after the target substance transfer step, precipitating a crystal of the target substance inside the second phase or at an interface between the first phase and the second phase.

9. The production method according to claim 8 ,
wherein in at least one of the target substance transfer step and the crystal precipitation step, at least one of the first phase and the second phase is stirred.

10. A method for producing a composition, the method comprising:
a target substance transfer step of transferring a target substance,
wherein the target substance transfer step is a step of transferring the target substance from the first phase to the second phase by the method according to claim 1 , and
the composition is a composition comprising the target substance in the second phase.

Assignees

• Mori Yusuke
• Inoue Tsuyoshi
• Takano Kazufumi
• Matsumura Hiroyoshi
• Adachi Hiroaki
• Sugiyama Shigeru
• Murai Ryota
• Kurata Masateru
• Yoshikawa Hiroshi
• Hirao Mihoko
• Nakayama Satoshi
• Takahashi Yoshinori
• Murakami Satoshi
• Univ Osaka

Inventors

• Mori Yusuke
• Inoue Tsuyoshi
• Takano Kazufumi
• Matsumura Hiroyoshi
• Adachi Hiroaki
• Sugiyama Shigeru
• Murai Ryota
• Kurata Masateru
• Yoshikawa Hiroshi
• Hirao Mihoko
• Nakayama Satoshi
• Takahashi Yoshinori
• Murakami Satoshi

Classifications

• B01D9/0081 Primary

  Use of vibrations, e.g. ultrasound · CPC title

• B01J19/121

  Coherent waves, e.g. laser beams (lasers per se H01S3/00) · CPC title

• B01J19/10

  employing sonic or ultrasonic vibrations · CPC title

• C30B29/58

  Macromolecular compounds · CPC title

• B01J19/008 Primary

  Processes for carrying out reactions under cavitation conditions · CPC title