Use of yeast flakes for treating and/or preventing hyperinsulinemia

Patent metadata

Field	Value
Publication number	US-9750762-B2
Application number	US-67043608-A
Country	US
Kind code	B2
Filing date	Jul 25, 2008
Priority date	Jul 25, 2007
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

The invention relates to the use of yeast flakes for preparing a pharmaceutical composition for treating and/or preventing hyperinsulinemia.

First claim

The invention claimed is:

1. A method of treatment of hyperinsulinemia in a patient in need thereof, comprising administering an effective amount of yeast cell walls to a non-hyperglycemic patient in need thereof, wherein said yeast cell walls have:
a.) a total content of glucan and mannan of at least 34.0% dry matter content by weight; and
b.) a glycogen content of less than 10.0% dry matter content by weight, wherein the non-hyperglycemic patient is overweight.

2. The method of claim 1 , wherein the yeast cell walls obtained from Saccharomyces cerevisiae.

3. The method of claim 1 , wherein said yeast cell walls have a total content of glucan and mannan of at least 40% dry matter content by weight.

4. The method of claim 1 , wherein said yeast cell walls have a mannan content of less than 30% dry matter content by weight.

5. The method of claim 1 , wherein said yeast cell walls have a mannan content of less than 2% dry matter content by weight.

6. The method of claim 1 , wherein said yeast cell walls have a total content of glucan and mannan of 90% or less dry matter content by weight.

7. The method of claim 1 , wherein said yeast cell walls have a total content of glucan and mannan of 45% to 90% dry matter content by weight.

8. The method of claim 1 , wherein said yeast cell walls have a glycogen content of less than 8% dry matter content by weight.

9. The method of claim 1 , wherein the yeast cell walls have a dry matter content of 90% or more dry matter content by weight.

10. The method of claim 1 , wherein said yeast cell walls have a total content of glucan and mannan of at least 45% dry matter content by weight.

11. The method of claim 1 , wherein said yeast cell walls have a mannan content less than 1% dry matter content by weight.

12. The method of claim 1 , wherein the yeast cell walls have a total content of glucan and mannan equal to or less than 80% dry matter content by weight.

13. The method of claim 1 , wherein the yeast cell walls have a total content of glucan and mannan of 52% to 80% dry matter content by weight.

14. The method of claim 1 , wherein the yeast cell walls have a glycogen content of less than 5% dry matter content by weight.

15. The method of claim 1 , wherein the yeast cell walls have a dry matter content of 94% or more.

16. The method of claim 1 , wherein said yeast cell walls are obtained by:
i.) submitting an aqueous suspension of yeast cells having a dry matter content between 12% and 18% by weight to hydrolysis using endogenous enzymes of said yeast cells and, optionally proteases exogenous to said yeast cells to provide an insoluble fraction;
ii.) separating the insoluble fraction; and
iii.) drying the separated insoluble fraction to obtain the said yeast cell walls, wherein the cells walls have a dry matter content of 90% by weight.

17. The method of claim 16 , wherein hydrolysis is conducted at a temperature of between 45° C. and 55° C. for 18 to 36 hours so as to solubilise at least 60% by weight of the dry matter of said yeast cells.

18. The method of claim 16 , wherein the drying step iii.) is performed by spray drying or drying on heated rolls.

19. The method of claim 16 , wherein the method further comprising:
iv.) heating the aqueous suspension of yeast cell walls of step i.) in an alkaline medium at a temperature of between 70° C. and 100° C. for no more than 3 hours; and
v.) separating the insoluble fraction produced to obtain the yeast cell walls, wherein said yeast cell walls are β-glucan-enriched; and,
and optionally:
vi.) submitting the separated insoluble fraction of step ii.) to acid treatment; and
vii.) collecting the insoluble fraction produced by acid treatment to obtain the yeast cell walls, wherein said yeast cell walls are β-glucan-enriched and devoid of mannans.

20. The method of claim 19 , wherein the suspension of yeast cell walls is heated at 85° C. for 2 hours in an alkaline sodium hydroxide aqueous medium.

21. The method of claim 19 , wherein the insoluble fraction produced by acid treatment is separated by centrifugation and washing, and optionally dried.

Assignees

• Laville Martine
• Nazare Julie-Anne
• Oriol Eric
• Lesaffre & Cie

Inventors

• Laville Martine
• Nazare Julie-Anne
• Oriol Eric

Classifications

• A61P3/08

  for glucose homeostasis (pancreatic hormones A61P5/48) · CPC title

• A61P3/04

  Anorexiants; Antiobesity agents · CPC title

• A61P3/06

  Antihyperlipidemics · CPC title

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61K31/736

  Glucomannans or galactomannans, e.g. locust bean gum, guar gum · CPC title