Substance-encapsulating vesicle and process for producing the same

Patent metadata

Field	Value
Publication number	US-9750687-B2
Application number	US-201113699238-A
Country	US
Kind code	B2
Filing date	May 23, 2011
Priority date	May 21, 2010
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

Provided is a method for easily and efficiently producing encapsulated substance vesicles wherein a substance is encapsulated in the cavity of vesicles obtained by polymer self-assembly. Empty vesicles that have membranes comprising a first polymer that is a block copolymer with uncharged hydrophilic segments and a first kind of charged segments and a second polymer with a second kind of charged segments that carry a charge that is the opposite of said first kind of charged segments as well as spaces that are enclosed by said membranes are mixed in an aqueous medium with the substance that is to be encapsulated in the spaces.

First claim

The invention claimed is:

1. A vesicle comprising:
a membrane containing a first polymer, which is a block copolymer having an uncharged hydrophilic segment and a first charged segment, and a second polymer, which has a second charged segment charged oppositely to the first charged segment, said membrane defining a cavity surrounded thereby and having a first charged segment to second charged segment ratio of 0.3 to less than 3.00; and
a charged substance encapsulated in the cavity.

2. A vesicle according to claim 1 , wherein the membrane has a trilaminar structure comprising an outer layer, an intermediate layer, and an inner layer.

3. A vesicle according to claim 1 , wherein the charged substance is selected from the group consisting of biomolecules, organic compounds and inorganic substances.

4. A vesicle according to claim 1 , wherein the first polymer is a block copolymer containing an uncharged hydrophilic segment and an anionic segment, and the second polymer is a block copolymer containing an uncharged hydrophilic segment and a cationic segment and/or a polymer containing a cationic segment but no uncharged hydrophilic segment.

5. A vesicle according to claim 1 , wherein the first polymer is a block copolymer containing an uncharged hydrophilic segment and a cationic segment, and the second polymer is a block copolymer containing an uncharged hydrophilic segment and an anionic segment and/or a polymer containing an anionic segment but no uncharged hydrophilic segment.

6. A vesicle according to claim 4 or 5 , wherein the anionic segment is nucleic acid.

7. A vesicle according to claim 4 or 5 , wherein the cationic segment is polyamine.

8. A vesicle according to claim 4 or 5 , wherein the uncharged hydrophilic segment is polyalkylene glycol.

Assignees

• Kataoka Kazunori
• Kishimura Akihiro
• Anraku Yasutaka
• Miyata Kanjiro
• Chuanoi Sayan
• Suma Tomoya
• Oba Makoto
• Japan Science & Tech Agency

Inventors

• Kataoka Kazunori
• Kishimura Akihiro
• Anraku Yasutaka
• Miyata Kanjiro
• Chuanoi Sayan
• Suma Tomoya
• Oba Makoto

Classifications

• A61K9/1273 Primary

  Polymersomes; Liposomes with polymerisable or polymerised bilayer-forming substances · CPC title

• B01J13/02

  Making microcapsules or microballoons {(for medical preparations A61K9/50)} · CPC title

• B01J13/14

  Polymerisation; cross-linking · CPC title

• A61K9/0087 Primary

  Galenical forms not covered by A61K9/02 - A61K9/7023 · CPC title