Systems and methods of detecting and demonstrating heat damage to hair via evaluation of peptides

Patent metadata

Field	Value
Publication number	US-9748085-B2
Application number	US-201514729699-A
Country	US
Kind code	B2
Filing date	Jun 3, 2015
Priority date	Jun 12, 2014
Publication date	Aug 29, 2017
Grant date	Aug 29, 2017

Abstract

A method to measure heat damage of keratin fibers comprising eluting a peptide from a hair sample with an aqueous solution; extracting the peptide using a suitable solvent sample; analyzing the peptide samples with MALDI-MS; resulting in peptide results; identifying presence of a marker peptide and identifying the m/z ratio for the peptide.

First claim

The invention claimed is:

1. A method to identify a treatment for heat damage of keratin fibers comprising
a) applying a treatment composition to a hair sample A; applying no treatment composition to hair sample B, wherein sample A and sample B proceed from the same subject;
b) applying heat to hair sample A and hair sample B;
c) eluting a peptide from the hair samples with an aqueous solution;
d) extracting the peptides using a suitable solvent sample;
e) measuring a marker peptide by MALDI-MS, identified by m/z 1061 for bleached hair or m/z of 1067 for virgin hair, wherein sample A has a decrease in measured peptide compared to sample B.

2. The method of claim 1 wherein the treatment composition comprises a peptide cross linking agent.

3. The method of claim 2 wherein the peptide cross linking agent is selected from the group consisting of sugars, carbonate esters, amines, diamines, carbonyl containing compounds, carboxylic acids, oxyethanoic acids and mixtures thereof.

4. The method of claim 1 wherein the treatment composition comprises a heat protecting agent.

5. The method of claim 4 wherein the heat protecting agent is selected from the group consisting of silicones, polymers, glycols, humectants, metal salts, oils, hydrolyzed keratin and mixtures thereof.

Assignees

• Procter & Gamble
• Procter & Gamble

Inventors

• Walker Ron
• Sun Yiping
• Davis Michael Glenn
• Washington Randy Purnell
• Beglinger Katherine Mae
• Davis Stephanie Lee

Classifications

• H01J49/0027

  Methods for using particle spectrometers · CPC title

• G01N33/6851 Primary

  Methods of protein analysis involving laser desorption ionisation mass spectrometry · CPC title

• H01J49/164 Primary

  Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418) · CPC title