In-situ pathogen detection using magnetoelastic sensors

Patent metadata

Field	Value
Publication number	US-9746443-B2
Application number	US-201314064308-A
Country	US
Kind code	B2
Filing date	Oct 28, 2013
Priority date	Oct 26, 2012
Publication date	Aug 29, 2017
Grant date	Aug 29, 2017

Abstract

In at least one illustrative embodiment, a method for in-situ pathogen detection may comprise distributing one or more magnetoelastic measurement sensors on a surface of a test object, wherein each of the one or more magnetoelastic measurement sensors includes a biorecognition element configured to bind with a pathogen to cause a shift in a characteristic frequency of the associated measurement sensor; applying a varying magnetic field, using a test coil, to the one or more magnetoelastic measurement sensors distributed on the surface of the test object, wherein the test object is positioned outside of an inner volume defined by the test coil; detecting a frequency response of the one or more magnetoelastic measurement sensors using the test coil, while applying the varying magnetic field; and determining whether the pathogen is present based on the detected frequency response of the one or more magnetoelastic measurement sensors.

First claim

The invention claimed is:

1. An in-situ pathogen detection system comprising:
one or more magnetoelastic measurement sensors each including a biorecognition element configured to bind with a pathogen to cause a shift in a characteristic frequency of the associated measurement sensor;
a test coil configured to (i) apply a uniform magnetic field to the one or more magnetoelastic measurement sensors, (ii) apply a varying magnetic field to the one or more magnetoelastic measurement sensors during application of the uniform magnetic field, and (iii) detect a frequency response of the one or more magnetoelastic measurement sensors to the applied varying magnetic field, while the one or more magnetoelastic measurement sensors are positioned on a surface of a test object that is outside of an inner volume defined by the test coil; and
a controller coupled to the test coil and configured to (i) transmit a first signal that causes the test coil to generate at least the varying magnetic field, (ii) receive a second signal indicative of the frequency response of the one or more magnetoelastic measurement sensors detected by the test coil, and (iii) determine whether the pathogen is present based on the second signal.

2. The in-situ pathogen detection system of claim 1 , wherein the test coil is a focused-field flat coil.

3. The in-situ pathogen detection system of claim 1 , wherein the test coil is further configured to apply the uniform magnetic field to align the one or more magnetoelastic measurement sensors prior to application of the varying magnetic field.

4. The in-situ pathogen detection system of claim 1 , further comprising one or more magnetoelastic control sensors that do not include the biorecognition element;
wherein the test coil is further configured to (i) apply the varying magnetic field to the one or more magnetoelastic control sensors and (ii) detect a frequency response of the one or more magnetoelastic control sensors to the applied varying magnetic field, while the one or more magnetoelastic control sensors are positioned on the surface of the test object; and
wherein the second signal is also indicative of the frequency response of the one or more magnetoelastic control sensors detected by the test coil.

5. The in-situ pathogen detection system of claim 4 , wherein the second signal is indicative of (i) a shifted characteristic frequency of the one or more magnetoelastic measurement sensors caused by binding of the biorecognition element with the pathogen and (ii) a control characteristic frequency of the one or more magnetoelastic control sensors.

6. The in-situ pathogen detection system of claim 5 , wherein the controller is configured to determine whether the pathogen is present by determining whether the shifted characteristic frequency is statistically significantly less than the control characteristic frequency.

7. The in-situ pathogen detection system of claim 1 , further comprising:
one or more additional magnetoelastic measurement sensors each including a different biorecognition element configured to bind with a different pathogen to cause a shift in a characteristic frequency of the associated additional measurement sensor;
wherein the test coil is further configured to (i) apply the varying magnetic field to the one or more additional magnetoelastic measurement sensors and (ii) detect a frequency response of the one or more additional magnetoelastic measurement sensors to the applied varying magnetic field, while the one or more additional magnetoelastic measurement sensors are positioned on the surface of the test object;
wherein the second signal is also indicative of the frequency response of the one or more additional magnetoelastic measurement sensors detected by the test coil; and
wherein the controller is further configured to determine whether the different pathogen is present based on the second signal.

8. The in-situ pathogen detection system of claim 1 , wherein the test coil comprises a number of loops surrounding the inner volume and a glass core positioned within the inner volume.

9. The in-situ pathogen detection system of claim 8 , wherein the test coil comprises a pair of partially overlapping flat coils having opposite winding directions.

10. The in-situ pathogen detection system of claim 9 , wherein the test coil comprises a first side and a second side, and wherein the one or more magnetoelastic measurement sensors are positioned adjacent to the first side of the test coil and a backing formed of a high magnetic permeability material is coupled to the second side of the test coil.

11. The in-situ pathogen detection system of claim 1 , wherein the test coil is configured to detect the frequency response by measuring a magnetic field generated by vibration of the one or more magnetoelastic measurement sensors in response to the applied varying magnetic field.

12. The in-situ pathogen detection system of claim 1 , wherein the biorecognition element comprises a bacteriophage that is genetically engineered to bind with the pathogen.

13. The in-situ pathogen detection system of claim 1 , wherein the first signal causes a frequency of the varying magnetic field generated by the test coil to vary through a range including the characteristic frequency of each of the one or more magnetoelastic measurement sensors when the biorecognition element has not bound with the pathogen.

14. The in-situ pathogen detection system of claim 13 , wherein the range includes one or more values that are one half of the characteristic frequency of each of the one or more magnetoelastic measurement sensors when the biorecognition element has not bound with the pathogen.

15. The in-situ pathogen detection system of claim 13 , wherein the second signal is indicative of an impedance of the test coil while varying the frequency of the varying magnetic field through the range.

16. The in-situ pathogen detection system of claim 1 , wherein the uniform magnetic field applied by the test coil is aligned with the varying magnetic field applied by the test coil.

17. The in-situ pathogen detection system of claim 1 , wherein the test coil comprises a permanent magnet configured to produce the uniform magnetic field.

18. The in-situ pathogen detection system of claim 1 , wherein the first signal further causes the test coil to generate the uniform magnetic field.

Assignees

• Chin Bryan A
• Cheng Zhongyang
• Li Suiqiong
• Park Mi-Kyung
• Horikawa Shin
• Chai Yating
• Weerakoon Kanchana
• Best Stevie R
• Baltazar-Lopez Martin E
• Wikle Howard C
• Univ Auburn

Inventors

• Chin Bryan A
• Cheng Zhongyang
• Li Suiqiong
• Park Mi-Kyung
• Horikawa Shin
• Chai Yating
• Weerakoon Kanchana
• Best Stevie R
• Baltazar-Lopez Martin E
• Wikle Howard C

Classifications

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title

• G01N27/72 Primary

  by investigating magnetic variables · CPC title

• G01N27/745 Primary

  for detecting magnetic beads used in biochemical assays (concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098) · CPC title