Methods and products for analyzing polymers

Patent metadata

Field	Value
Publication number	US-9745625-B2
Application number	US-201314034407-A
Country	US
Kind code	B2
Filing date	Sep 23, 2013
Priority date	Feb 12, 1997
Publication date	Aug 29, 2017
Grant date	Aug 29, 2017

Abstract

Methods and products for analyzing polymers are provided. The methods include methods for determining various other structural properties of the polymers.

First claim

The invention claimed is:

1. A system for analyzing biological molecules, comprising:
a device having a plurality of passageways; and
a molecular motor in communication with at least one of the passageways, the molecule motor configured to receive a biological molecule, and further configured to move the biological molecule at least partially through the at least one passageway, wherein the at least one passageway is a nanopore or a nanochannel.

2. The system of claim 1 , wherein the at least one passageway is a nanopore.

3. The system of claim 1 , wherein the at least one passageway is a nanochannel.

4. The system of claim 1 , wherein each of the plurality of passageways is in communication with a molecular motor, and each molecular motor is configured to receive a distinct biological molecule, and each molecular motor is configured to move the distinct biological molecules through distinct passageways.

5. The system of claim 4 , wherein the passageways are nanopores.

6. The system of claim 1 , wherein the molecular motor is a polymerase.

7. The system of claim 1 , wherein the molecular motor is a helicase.

8. The system of claim 1 , wherein the biological molecule is DNA.

9. The system of claim 8 , wherein the biological molecule is single-stranded DNA.

10. The system of claim 1 , wherein the molecular motor is configured to linearly thread the biological molecule through the at least one passageway.

11. The system of claim 1 , further comprising a station, the station configured to produce a detectable signal as the biological molecule moves relative to the station.

12. The system of claim 11 , wherein the station undergoes a physical change as the biological molecule moves relative to the station, the physical change producing the detectable signal.

13. The system of claim 11 , wherein the detectable signal is a conductance measured across the passageway.

14. The system of claim 11 , wherein the detectable signal is a resistance measured across the passageway.

15. The system of claim 11 , wherein the station is at least a portion of the at least one passageway.

16. The system of claim 11 , wherein the biological molecule is single-stranded DNA, and the detectable signals relate to a nucleotide sequence of the single-stranded DNA.

17. The system of claim 1 , wherein the molecular motor ratchets the biological molecule through the at least one passageway.

18. A DNA sequencing system, comprising:
a plurality of molecular motors, each molecular motor corresponding to a distinct nanopore, each molecular motor configured to receive a distinct DNA molecule, and further configured to thread the distinct DNA molecule through the corresponding distinct nanopore; and
at least one detector configured to detect at least one signal from at least one nanopore as the distinct DNA molecule moves relative to the distinct nanopore, the at least one signal relating to a sequence of nucleotides of the distinct DNA molecule.

19. The system of claim 18 , wherein the DNA molecule is a single-stranded DNA molecule, and the molecular motor is a polymerase.

20. A biological analysis system, comprising:
a plurality of molecular motors, each molecular motor corresponding to a distinct nanopore of a device, each molecular motor configured to receive a distinct DNA molecule, and further configured to thread the distinct DNA molecule through the corresponding distinct nanopore.

Assignees

• Life Technologies Corp

Inventors

• Chan Eugene Y

Classifications

• G01N21/6428

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• G01N21/05

  Flow-through cuvettes (G01N21/09 takes precedence; handling fluid samples G01N1/10) · CPC title

• G01N2021/6441

  with two or more labels · CPC title

• C12Q1/6809

  Methods for determination or identification of nucleic acids involving differential detection · CPC title

• G01N2021/0346

  Capillary cells; Microcells · CPC title