Microalgae aurantiochytrium sp. LA3 (KCTC12685BP) and method for preparing bio-oil using the same

Patent metadata

Field	Value
Publication number	US-9745606-B2
Application number	US-201514813689-A
Country	US
Kind code	B2
Filing date	Jul 30, 2015
Priority date	Oct 8, 2014
Publication date	Aug 29, 2017
Grant date	Aug 29, 2017

Abstract

Provided herein are microalgae of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Aurantiochytrium sp. LA3 (KCTC12685BP) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 unsaturated fatty acids of 30% by weight or more based on total fatty acids, characterized by culturing the microalgae. The microalgae Aurantiochytrium sp. LA3 (KCTC12685BP) described herein has a rapid sugar consumption rate when being cultured using glucose as a carbon source, has a high oil content, allows cells to be cultured at a high concentration, and allows oil to be obtained in high productivity and a high yield, and thus, may produce bio-oil more economically and environmentally friendly.

First claim

The invention claimed is:

1. A method of preparing bio-oil, the method comprising the steps of:
(1) culturing microalgae Aurantiochytrium sp. LA3 (KCTC 12685BP); and
(2) extracting and separating bio-oil containing omega-3 unsaturated fatty acid from the cultured microalgae.

2. The method of claim 1 , wherein the bio-oil comprises 30 wt % or more omega-3 unsaturated fatty acid based on total fatty acids.

3. The method of claim 1 , wherein the culturing of step (1) is carried out in a manner selected from the group consisting of batchwise, fed-batchwise, and continuous culturing.

4. The method of claim 1 , wherein step (2) further comprises a cell disruption step.

5. The method of claim 4 , wherein the cell disruption is carried out in a manner selected from the group consisting of a supersonic disperser, a pulsed electric field, an enzyme, osmotic pressure, an electron beam, and an organic solvent.

6. The method of claim 1 , further comprising the step of (3) purifying bio-oil containing the omega-3 fatty acid.

7. The method of claim 6 , wherein the purification comprises collecting only an oil phase among an oil phase containing bio-oil and an aqueous phase containing cell pieces.

8. The method of claim 6 , wherein the purification comprises one or more steps selected from the group consisting of removing a solidified oil fraction, bleaching using bleaching clay or activated carbon, filtering, and deodorizing.

9. The method of claim 8 , wherein the deodorizing is carried out by a steam deodorizing process under reduced pressure.

Assignees

• Sk Innovation Co Ltd

Inventors

• Park Joong Min
• Kim Chang Kuk
• La Yeon Hwa
• Seo Young Bin

Classifications

• C11B1/025

  by enzymes or microorganisms, living or dead · CPC title

• C11B1/10

  by extracting · CPC title

• C12P7/6409 Primary

  Fatty acids · CPC title

• C11B7/0075

  by differences of melting or solidifying points · CPC title

• C11B3/14

  with the use of indifferent gases or vapours, e.g. steam · CPC title