Nanocrystalline cellulose hydrogels for inhibition of bacterial adhesion

Patent metadata

Field	Value
Publication number	US-9744270-B2
Application number	US-201414898944-A
Country	US
Kind code	B2
Filing date	Jun 20, 2014
Priority date	Jun 20, 2013
Publication date	Aug 29, 2017
Grant date	Aug 29, 2017

Abstract

The invention provides compositions for the inhibition of bacterial adhesion. The composition can include nanocrystalline cellulose (NCC) and a water-soluble polymer, which form an NCC hydrogel in water. The NCC hydrogel can be used to treat or coat devices such as a catheter, whereby the NCC hydrogel inhibits bacterial adhesion to the catheter surface to thereby inhibit biofilm formation and growth of bacteria on the catheter surface. The compositions and devices can thus be useful for inhibiting or preventing conditions such as urinary tract infections.

First claim

What is claimed is:

1. A catheter comprising a nanocrystalline cellulose (NCC) hydrogel coating for the inhibition of urinary tract infections, wherein the NCC hydrogel comprises NCC, a water-soluble polymer, and water, wherein the NCC hydrogel comprises about 1-20 wt. % NCC with respect to the mass of the water-soluble polymer, whereby the NCC hydrogel inhibits bacterial adhesion to the catheter surface to thereby inhibit biofilm formation and inhibit growth of bacteria on the catheter surface.

2. The catheter of claim 1 wherein the catheter surface comprises silica, silicone, polyvinyl chloride, or latex rubber.

3. The catheter of claim 1 wherein the NCC hydrogel further comprises humic acid or a saccharide.

4. The catheter of claim 1 wherein the NCC and water-soluble polymer comprise about 0.1 wt. % to about 10 wt. % of the mass of the NCC hydrogel.

5. The catheter of claim 1 wherein the polymer is a hydroxyalkyl cellulose or a carboxyalkyl cellulose.

6. The catheter of claim 5 wherein the polymer is 2-hydroxyethyl cellulose (HEC) or carboxymethyl cellulose (CMC).

7. The catheter of claim 1 wherein the pH of the NCC hydrogel is about 5.2 to about 7.4.

8. The catheter of claim 1 wherein the ionic strength of the NCC hydrogel is about 5 mM to about 50 mM.

9. A device for preventing catheter associated urinary tract infections on a urinary catheter comprising a catheter coated with a nanocrystalline cellulose (NCC) hydrogel, wherein the NCC hydrogel comprises NCC, a water-soluble polymer, and water; the NCC hydrogel comprises about 1-20 wt. % NCC with respect to the mass of the water-soluble polymer; the pH of the NCC hydrogel is about 6.2 to about 7.3; and the ionic strength of the NCC hydrogel is about 5 mM to about 40 mM.

10. A method of reducing bacterial adhesion to a surface of a medical device comprising contacting bacteria on or in proximity to a surface with nanocrystalline cellulose (NCC), wherein the bacteria are bacteria that produce high amounts of extracellular polymeric substance (EPS), thereby causing flocculation or aggregation, and thereby reducing the adhesion of the bacteria to the surface.

11. The method of claim 10 wherein the bacteria that produce high amounts of extracellular polymeric substance (EPS) comprise Pseudomonas fluorescence, Pseudomonas aeruginosa, Salmonella spp., or Klebsiella pneumoniae.

12. The method of claim 10 wherein the bacteria are in a dispersion and form flocs or aggregates in the presence of the nanocrystalline cellulose (NCC).

13. The method of claim 10 wherein the nanocrystalline cellulose (NCC) is present in a coating, film, textile, or reinforcing filler.

14. The method of claim 10 wherein the surface is a silica surface, a silicone surface, a polyvinyl chloride surface, or a latex rubber surface.

15. The method of claim 10 wherein the nanocrystalline cellulose (NCC) is in the form of a hydrogel comprising NCC, a water-soluble polymer, and water, and humic acid or a monosaccharide.

16. The method of claim 15 wherein the hydrogel comprises about 1-20 wt. % NCC with respect to the mass of the water-soluble polymer.

17. A hydrogel composition comprising nanocrystalline cellulose (NCC), a water-soluble polymer, water, and one or both of humic acid and a saccharide, wherein the hydrogel comprises about 1-20 wt. % NCC with respect to the mass of the water-soluble polymer, the NCC and water-soluble polymer comprise about 0.1 wt. % to about 10 wt. % of the mass of the NCC hydrogel, and the humic acid or saccharide is present in a concentration of about 0.1 mg/mL to about 10 mg/mL.

18. The catheter of claim 1 wherein the hydrogel coating comprises nanocrystalline cellulose (NCC), a water-soluble polymer, water, and one or both of humic acid and a saccharide, wherein the hydrogel comprises about 1-20 wt. % NCC with respect to the mass of the water-soluble polymer, the NCC and water-soluble polymer comprise about 0.1 wt. % to about 10 wt. % of the mass of the NCC hydrogel, and the humic acid or saccharide is present in a concentration of about 0.1 mg/mL to about 10 mg/mL.

Assignees

• Univ Alberta

Inventors

• Boluk Yaman
• Liu Yang
• Sun Xiaohui

Classifications

• A61L29/085 Primary

  Macromolecular materials · CPC title

• C08L1/00

  Compositions of cellulose, modified cellulose or cellulose derivatives · CPC title

• A61L29/041

  obtained by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• A61L29/145

  Hydrogels or hydrocolloids · CPC title

• C08J2301/02

  Cellulose; Modified cellulose · CPC title