HUMANIZED IL-4 AND IL-4Ra ANIMALS
US-2015320021-A1 · Nov 12, 2015 · US
Humanized IL-4 and IL-4Ra animals
US9743647B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9743647-B2 |
| Application number | US-201514791978-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 6, 2015 |
| Priority date | May 7, 2014 |
| Publication date | Aug 29, 2017 |
| Grant date | Aug 29, 2017 |
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Abstract
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Non-human animals comprising a human or humanized IL-4 and/or IL-4Rα nucleic acid sequence are provided. Non-human animals that comprise a replacement of the endogenous IL-4 gene and/or IL-4Rα gene with a human IL-4 gene and/or IL-4Rα gene in whole or in part, and methods for making and using the non-human animals, are described. Non-human animals comprising a human or humanized IL-4 gene under control of non-human IL-4 regulatory elements is also provided, including non-human animals that have a replacement of non-human IL-4-encoding sequence with human IL-4-encoding sequence at an endogenous non-human IL-4 locus. Non-human animals comprising a human or humanized IL-4Rα gene under control of non-human IL-4Rα regulatory elements is also provided, including non-human animals that have a replacement of non-human IL-4Rα-encoding sequence with human or humanized IL-4Rα-encoding sequence at an endogenous non-human C IL-4Rα locus. Non-human animals comprising human or humanized IL-4 gene and/or IL-4Rα sequences, wherein the non-human animals are rodents, e.g., mice or rats, are provided.
First claim
Opening claim text (preview).
What is claimed is: 1. A rodent, comprising a replacement of a genomic DNA of a rodent IL-4Rα gene at an endogenous rodent IL-4Rα locus with a human genomic DNA of a human IL-4Rα gene to form a humanized IL-4Rα gene, wherein the genomic DNA of the rodent IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the rodent IL-4Rα gene, and the human genomic DNA of the human IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene; wherein the humanized IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene and exons 6-9 of the rodent IL-4Rα gene, and the human genomic DNA present in the humanized IL-4Rα gene begins with the ATG initiation codon in exon 1 of the human IL-4Rα gene; and wherein expression of the humanized IL-4Rα gene is under control of the rodent IL-4Rα promoter at the endogenous rodent IL-4Rα locus, wherein the rodent is a mouse or a rat. 2. The rodent of claim 1 , wherein the rodent is a mouse that is incapable of expressing a mouse IL-4Rα protein. 3. The rodent of claim 1 , wherein the rodent expresses a human or humanized IL-4 protein. 4. The rodent of claim 3 , wherein the rodent comprises a replacement of a genomic DNA of a rodent IL-4 gene at an endogenous rodent IL-4 locus with a genomic DNA of a human IL-4 gene to form a humanized IL-4 gene, wherein the genomic DNA of the rodent IL-4 gene comprises the ATG initiation codon of exon 1 through exon 4 of the rodent IL-4 gene, and the genomic DNA of the human IL-4 gene comprises the ATG initiation codon of exon 1 through exon 4 of the human IL-4 gene, and wherein expression of the humanized IL-4 gene is under control of the rodent IL-4 promoter at the endogenous rodent IL-4 locus. 5. The rodent of claim 1 , wherein the rodent is a mouse. 6. A method for making a humanized rodent, comprising replacing a genomic DNA of a rodent IL-4Rα gene at an endogenous rodent IL-4Rα locus with a human genomic DNA of a human IL-4Rα gene to form a humanized IL-4Rα gene, wherein the genomic DNA of the rodent IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the rodent IL-4Rα gene, and the human genomic DNA of the human IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene; wherein the humanized IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene followed by exons 6-9 of the rodent IL-4Rα gene, and the human genomic DNA present in the humanized IL-4Rα gene begins with the ATG initiation codon of exon 1 of the human IL-4Rα gene; and wherein the humanized IL-4Rα gene is operably linked to the rodent IL-4Rα promoter at the endogenous rodent IL-4Rα locus, wherein the rodent is a mouse or a rat. 7. The method of claim 6 , wherein the rodent is a mouse. 8. The rodent of claim 3 , wherein the rodent is a mouse that does not express a mouse IL-4 protein.
Assignees
Inventors
Classifications
IL-4 · CPC title
for interleukins [IL] · CPC title
Murine · CPC title
Animal expressing industrially exogenous proteins · CPC title
Animal model comprising a reporter system for screening tests · CPC title
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Frequently asked questions
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- What does patent US9743647B2 cover?
- Non-human animals comprising a human or humanized IL-4 and/or IL-4Rα nucleic acid sequence are provided. Non-human animals that comprise a replacement of the endogenous IL-4 gene and/or IL-4Rα gene with a human IL-4 gene and/or IL-4Rα gene in whole or in part, and methods for making and using the non-human animals, are described. Non-human animals comprising a human or humanized IL-4 gene under…
- Who is the assignee on this patent?
- Regeneron Pharma
- What technology area does this patent fall under?
- Primary CPC classification A01K67/0278. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Aug 29 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).